HMM Summary Page: TIGR03444

Functionergothioneine biosynthesis glutamate--cysteine ligase EgtA
Gene SymbolegtA
Trusted Cutoff370.80
Domain Trusted Cutoff370.80
Noise Cutoff278.85
Domain Noise Cutoff278.85
Isology Typeequivalog
EC Number6.3.2.2
HMM Length390
Mainrole CategoryUnknown function
Subrole CategoryEnzymes of unknown specificity
AuthorHaft DH
Entry DateJun 27 2007 1:06PM
Last ModifiedJun 21 2012 2:17PM
CommentMembers of this bacterial protein family, EgtA, resemble the glutamate--cysteine ligase of the two-step pathway for glutathione (GSH) biosynthesis, but instead are involved in the biosynthesis of the histidine-derived thiol, ergothioneine (EGT). Successful in vitro reconstitution of EGT biosynthesis using EgtBCDE and gamma-L-glutamyl-L-cysteine suggests that this enzyme is a bone fide glutamate--cysteine ligase.
ReferencesRN [1] RM PMID:20420449 RT In vitro reconstitution of Mycobacterial ergothioneine biosynthesis. RA Seebeck FP RL J Am Chem Soc. 2010 May 19;132(19):6632-3.
Genome PropertyGenProp0752: ergothioneine biosynthesis (HMM)