HMM Summary Page: TIGR04365

Functionautonomous glycyl radical cofactor GrcA
Gene SymbolgrcA
Trusted Cutoff170.00
Domain Trusted Cutoff170.00
Noise Cutoff160.00
Domain Noise Cutoff160.00
Isology Typeequivalog
HMM Length126
AuthorHaft DH
Entry DateAug 5 2012 5:13PM
Last ModifiedAug 12 2012 6:56PM
CommentThis small protein, previously designated YfiD in E. coli, is closely homologous to pyruvate formate_lyase (PFL) in a region surrounding the stable glycyl radical that is prepared by the action of pyruvate formate-lyase activase, a radical SAM enzyme. When damage at the site of this radical breaks the main chain of PFL, this protein acts as a spare part that reintroduces the needed stable glycyl radical. Cutoffs for this model are set to exclude a set of closely related phage proteins that appear to have a corresponding function.
ReferencesRN [1] RM PMID:11444864 RT YfiD of Escherichia coli and Y06I of bacteriophage T4 as autonomous glycyl radical cofactors reconstituting the catalytic center of oxygen-fragmented pyruvate formate-lyase. RA Wagner AF, Schultz S, Bomke J, Pils T, Lehmann WD, Knappe J RL Biochem Biophys Res Commun. 2001 Jul 13;285(2):456-62.
Genome PropertyGenProp0943: pyruvate formate-lyase system (HMM)