HMM Summary Page: TIGR04266

FunctionNDMA-dependent methanol dehydrogenase
Gene Symbolmdo
Trusted Cutoff625.00
Domain Trusted Cutoff625.00
Noise Cutoff400.00
Domain Noise Cutoff400.00
Isology Typeequivalog
EC Number1.1.99.37
HMM Length420
AuthorHaft DH
Entry DateJan 16 2012 9:35AM
Last ModifiedApr 28 2014 12:11PM
CommentMembers of this family belong to the iron-dependent alcohol dehydrogenase family (see PF00465). The NADP(H) cofactor is bound too tightly for exchange (although non-convalently), so enzymatic activity depends on a second substrate or electron carrier. The radical SAM-modified natural product mycofactocin is proposed to fill this role. In Rhodococcus erythropolis N9T-4, a role was shown for this protein in CO2 fixation during extreme oligotrophic (or possibly chemoautotrophic) growth.
ReferencesRN [1] RM PMID:8449887 RT Electron microscopic analysis and structural characterization of novel NADP(H)-containing methanol: N,N'-dimethyl-4-nitrosoaniline oxidoreductases from the gram-positive methylotrophic bacteria Amycolatopsis methanolica and Mycobacterium gastri MB19. RA Bystrykh LV, Vonck J, van Bruggen EF, van Beeumen J, Samyn B, Govorukhina NI, Arfman N, Duine JA, Dijkhuizen L RL J Bacteriol. 1993 Mar;175(6):1814-22. RN [2] RM PMID:21223593 RT Bioinformatic evidence for a widely distributed, ribosomally produced electron carrier precursor, its maturation proteins, and its nicotinoprotein redox partners. RA Haft DH RL BMC Genomics. 2011 Jan 11;12:21. RN [3] RM PMID:17675378 RT An extremely oligotrophic bacterium, Rhodococcus erythropolis N9T-4, isolated from crude oil. RA Ohhata N, Yoshida N, Egami H, Katsuragi T, Tani Y, Takagi H RL J Bacteriol. 2007 Oct;189(19):6824-31.
Genome PropertyGenProp0917: mycofactocin system (HMM)