HMM Summary Page: TIGR04085

Functionradical SAM additional 4Fe4S-binding SPASM domain
Trusted Cutoff27.00
Domain Trusted Cutoff27.00
Noise Cutoff16.00
Domain Noise Cutoff16.00
Isology Typedomain
HMM Length93
AuthorHaft DH
Entry DateNov 5 2010 1:36PM
Last ModifiedAug 25 2011 2:00PM
CommentThis domain contains regions binding additional 4Fe4S clusters found in various radical SAM proteins C-terminal to the domain described by model PF04055. Radical SAM enzymes with this domain tend to be involved in protein modification, including anaerobic sulfatase maturation proteins, a quinohemoprotein amine dehydrogenase biogenesis protein, the Pep1357-cyclizing radical SAM enzyme, and various bacteriocin biosynthesis proteins. The motif CxxCxxxxxCxxxC is nearly invariant for members of this family, although PqqE has a variant form. We name this domain SPASM for Subtilosin, PQQ, Anaerobic Sulfatase, and Mycofactocin.
ReferencesRN [1] RM PMID:21478363 RT Biological systems discovery in silico: radical S-adenosylmethionine protein families and their target peptides for posttranslational modification. RA Haft DH, Basu MK RL J Bacteriol. 2011 Jun;193(11):2745-55. Epub 2011 Apr 8. RN [1] RM PMID:21223593 RT Bioinformatic evidence for a widely distributed, ribosomally produced electron carrier precursor, its maturation proteins, and its nicotinoprotein redox partners. RA Haft DH RL BMC Genomics. 2011 Jan 11;12:21. RN [3] RM PMID:20218986 RT Anaerobic sulfatase-maturating enzyme--a mechanistic link with glycyl radical-activating enzymes? RA Benjdia A, Subramanian S, Leprince J, Vaudry H, Johnson MK, Berteau O RL FEBS J. 2010 Apr;277(8):1906-20. Epub 2010 Mar 9.