| Accession | TIGR04075 |
| Name | bacter_Pnkp |
| Function | polynucleotide kinase-phosphatase |
| Trusted Cutoff | 750.00 |
| Domain Trusted Cutoff | 750.00 |
| Noise Cutoff | 550.00 |
| Domain Noise Cutoff | 550.00 |
| Isology Type | equivalog |
| HMM Length | 851 |
| Author | Haft DH |
| Entry Date | Oct 17 2010 9:20AM |
| Last Modified | Feb 14 2011 3:27PM |
| Comment | Members of this protein family are the bacterial polynucleotide kinase-phosphatase (Pnkp) whose genes occur paired with genes for the 3' terminal RNA ribose 2'-O-methyltransferase Hen1. All members of the seed alignment belong to a cassette with the Hen1. The pair acts in bacterial RNA repair. This enzyme performs end-healing reactions on broken RNA, preparing from the RNA ligase to close the break. The working hypothesis is that the combination of Pnkp (RNA repair) and Hen1 (RNA modification) serves to first repair RNA damage from ribotoxins and then perform a modification that prevents the damage from recurring. |
| References | RN [1]
RM PMID:17986465
RT Characterization of the 2',3' cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase.
RA Keppetipola N, Shuman S
RL Nucleic Acids Res. 2007;35(22):7721-32. Epub 2007 Nov 5.
RN [2]
RM PMID:20007328
RT Bacterial Hen1 is a 3' terminal RNA ribose 2'-O-methyltransferase component of a bacterial RNA repair cassette.
RA Jain R, Shuman S
RL RNA. 2010 Feb;16(2):316-23. |
| Genome Property | GenProp0948: RNA repair, Hen1/Pnkp system (HMM) |
