HMM Summary Page: TIGR03814

Functionglutaminase A
Gene SymbolglsA
Trusted Cutoff255.40
Domain Trusted Cutoff255.40
Noise Cutoff246.00
Domain Noise Cutoff246.00
Isology Typeequivalog
EC Number3.5.1.2
HMM Length300
Mainrole CategoryEnergy metabolism
Subrole CategoryAmino acids and amines
Gene Ontology TermGO:0004359: glutaminase activity molecular_function
GO:0006541: glutamine metabolic process biological_process
AuthorHaft DH
Entry DateSep 2 2009 12:03PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis family describes the enzyme glutaminase, from a larger family that includes serine-dependent beta-lactamases and penicillin-binding proteins. Many bacteria have two isozymes. This model is based on selected known glutaminases and their homologs within prokaryotes, with the exclusion of highly-derived (long branch) and architecturally varied homologs, so as to achieve conservative assignments. A sharp drop in scores occurs below 250, and cutoffs are set accordingly. The enzyme converts glutamine to glutamate, with the release of ammonia. Members tend to be described as glutaminase A (glsA), where B (glsB) is unknown and may not be homologous (as in Rhizobium etli). Some species have two isozymes that may both be designated A (GlsA1 and GlsA2).
ReferencesRN [1] RM PMID:10095071 RT Sequence and molecular analysis of the Rhizobium etli glsA gene, encoding a thermolabile glutaminase. RA Calderon J, Huerta-Saquero A, Du Pont G, Duran S RL Biochim Biophys Acta. 1999 Mar 19;1444(3):451-6. RN [2] RM PMID:18459799 RT Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis. RA Brown G, Singer A, Proudfoot M, Skarina T, Kim Y, Chang C, Dementieva I, Kuznetsova E, Gonzalez CF, Joachimiak A, Savchenko A, Yakunin AF RL Biochemistry. 2008 May 27;47(21):5724-35.