HMM Summary Page: TIGR03723

FunctiontRNA threonylcarbamoyl adenosine modification protein TsaD
Gene SymboltsaD
Trusted Cutoff296.10
Domain Trusted Cutoff296.10
Noise Cutoff287.85
Domain Noise Cutoff287.85
Isology Typeequivalog
HMM Length314
Mainrole CategoryUnknown function
Subrole CategoryGeneral
AuthorHaft DH
Entry DateMar 18 2009 10:07AM
Last ModifiedAug 12 2012 6:56PM
CommentThis model represents bacterial members of a protein family that is widely distributed. In a few pathogenic species, the protein is exported in a way that may represent an exceptional secondary function. This model plus companion (archaeal) model TIGR03722 together span the prokaryotic member sequences of TIGR00329, a protein family that appears universal in life, and whose broad function is unknown. A member of TIGR03722 has been characterized as a DNA-binding protein with apurinic endopeptidase activity. In contrast, the rare characterized members of the present family show O-sialoglycoprotein endopeptidase (EC. activity after export. These include glycoprotease (gcp) from Pasteurella haemolytica A1 and a cohemolysin from Riemerella anatipestifer (GB|AAG39646.1). The member from Staphylococcus aureus is essential and is related to cell wall dynamics and the modulation of autolysis, but members are also found in the Mycoplasmas (which lack a cell wall). A reasonable hypothesis is that virulence-related activities after export are secondary to a bacterial domain-wide unknown function.
ReferencesRN [1] RM PMID:22378793 RT Biosynthesis of Threonylcarbamoyl Adenosine (t6A), a Universal tRNA Nucleoside. RA Deutsch C, El Yacoubi B, de Crecy-Lagard V, Iwata-Reuyl D RL J Biol Chem. 2012 Apr 20;287(17):13666-73. RN [2] RM PMID:17237169 RT Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus. RA Zheng L, Yu C, Bayles K, Lasa I, Ji Y RL J Bacteriol. 2007 Apr;189(7):2734-42. RN [3] RM PMID:1885539 RT Cloning, nucleotide sequence, and expression of the Pasteurella haemolytica A1 glycoprotease gene. RA Abdullah KM, Lo RY, Mellors A RL J Bacteriol. 1991 Sep;173(18):5597-603.
Genome PropertyGenProp1060: tRNA N6-threonylcarbamoyladenosine modification (HMM)
GenProp0799: bacterial core gene set, exactly 1 per genome (HMM)