HMM Summary Page: TIGR03686

FunctionPup--protein ligase
Gene SymbolpafA
Trusted Cutoff551.30
Domain Trusted Cutoff551.30
Noise Cutoff429.85
Domain Noise Cutoff429.85
Isology Typeequivalog
EC Number6.3.2.-
HMM Length457
Mainrole CategoryProtein fate
Subrole CategoryDegradation of proteins, peptides, and glycopeptides
Gene Ontology TermGO:0010498: proteasomal protein catabolic process biological_process
GO:0016879: ligase activity, forming carbon-nitrogen bonds molecular_function
GO:0019941: modification-dependent protein catabolic process biological_process
AuthorHaft DH
Entry DateNov 5 2008 9:39AM
Last ModifiedFeb 14 2011 3:27PM
CommentMembers of this family are the Pup--protein ligase PafA (proteasome accessory factor A), a protein shown to regulate steady-state levels of certain proteasome targets in Mycobacterium tuberculosis. Iyer, et al (2008) first suggested that PafA is the ligase for Pup, a ubiquitin analog attached to an epsilon-amino group of a Lys side-chain to direct the target to the proteasome.
ReferencesRN [1] RM PMID:17277063 RT Characterization of the proteasome accessory factor (paf) operon in Mycobacterium tuberculosis. RA Festa RA, Pearce MJ, Darwin KH RL J Bacteriol. 2007 Apr;189(8):3044-50. RN [2] RM PMID:22910360 RT Structures of Pup ligase PafA and depupylase Dop from the prokaryotic ubiquitin-like modification pathway. RA Ozcelik D, Barandun J, Schmitz N, Sutter M, Guth E, Damberger FF, Allain FH, Ban N, Weber-Ban E RL Nat Commun. 2012;3:1014. doi: 10.1038/ncomms2009.
Genome PropertyGenProp0833: proteasome-targeting modification by pupylation (HMM)