HMM Summary Page: TIGR03414

Functioncholine ABC transporter, periplasmic binding protein
Gene SymbolchoX
Trusted Cutoff379.05
Domain Trusted Cutoff379.05
Noise Cutoff191.50
Domain Noise Cutoff191.50
Isology Typesubfamily
HMM Length291
Mainrole CategoryTransport and binding proteins
Subrole CategoryAmino acids, peptides and amines
Gene Ontology TermGO:0015220: choline transmembrane transporter activity molecular_function
GO:0015871: choline transport biological_process
GO:0055052: ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing cellular_component
AuthorHaft DH
Entry DateMay 24 2007 12:06AM
Last ModifiedFeb 14 2011 3:27PM
CommentPartial phylogenetic profiling (PMID:16930487) vs. the genome property of glycine betaine biosynthesis from choline consistently reveals a member of this ABC transporter periplasmic binding protein as the best match, save for the betaine biosynthesis enzymes themselves. Genomes often carry several paralogs, one encoded together with the permease and ATP-binding components and another encoded next to a choline-sulfatase gene, suggesting that different members of this protein family interact with shared components and give some flexibility in substrate. Of two members from Sinorhizobium meliloti 1021, one designated ChoX has been shown experimentally to bind choline (though not various related compounds such as betaine) and to be required for about 60 % of choline uptake. Members of this protein have an invariant Cys residue near the N-terminus and likely are lipoproteins.
ReferencesRN [1] RM PMID:15342567 RT The Sinorhizobium meliloti ABC transporter Cho is highly specific for choline and expressed in bacteroids from Medicago sativa nodules. RA Dupont L, Garcia I, Poggi MC, Alloing G, Mandon K, Le Rudulier D. RL J Bacteriol. 2004 Sep;186(18):5988-96. RN [2] RM PMID: 16930487 RT Exopolysaccharide-associated protein sorting in environmental organisms: the PEP-CTERM/EpsH system. Application of a novel phylogenetic profiling heuristic. RA Haft DH, Paulsen IT, Ward N, Selengut JD RL BMC Biol. 2006 Aug 24;4:29.
Genome PropertyGenProp0748: choline ABC transporter, ChoXWV family (HMM-CLUST)
GenProp0749: choline sulfate utilization (HMM-CLUST)