| Accession | TIGR03414 |
| Name | ABC_choline_bnd |
| Function | choline ABC transporter, periplasmic binding protein |
| Gene Symbol | choX |
| Trusted Cutoff | 379.05 |
| Domain Trusted Cutoff | 379.05 |
| Noise Cutoff | 191.50 |
| Domain Noise Cutoff | 191.50 |
| Isology Type | subfamily |
| HMM Length | 291 |
| Mainrole Category | Transport and binding proteins |
| Subrole Category | Amino acids, peptides and amines |
| Gene Ontology Term | GO:0015220: choline transmembrane transporter activity molecular_function |
| | GO:0015871: choline transport biological_process |
| | GO:0055052: ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing cellular_component |
| Author | Haft DH |
| Entry Date | May 24 2007 12:06AM |
| Last Modified | Feb 14 2011 3:27PM |
| Comment | Partial phylogenetic profiling (PMID:16930487) vs. the genome property of glycine betaine biosynthesis from choline consistently reveals a member of this ABC transporter periplasmic binding protein as the best match, save for the betaine biosynthesis enzymes themselves. Genomes often carry several paralogs, one encoded together with the permease and ATP-binding components and another encoded next to a choline-sulfatase gene, suggesting that different members of this protein family interact with shared components and give some flexibility in substrate. Of two members from Sinorhizobium meliloti 1021, one designated ChoX has been shown experimentally to bind choline (though not various related compounds such as betaine) and to be required for about 60 % of choline uptake. Members of this protein have an invariant Cys residue near the N-terminus and likely are lipoproteins. |
| References | RN [1]
RM PMID:15342567
RT The Sinorhizobium meliloti ABC transporter Cho is highly specific for choline and expressed in bacteroids from Medicago sativa nodules.
RA Dupont L, Garcia I, Poggi MC, Alloing G, Mandon K, Le Rudulier D.
RL J Bacteriol. 2004 Sep;186(18):5988-96.
RN [2]
RM PMID: 16930487
RT Exopolysaccharide-associated protein sorting in environmental organisms: the PEP-CTERM/EpsH system. Application of a novel phylogenetic profiling heuristic.
RA Haft DH, Paulsen IT, Ward N, Selengut JD
RL BMC Biol. 2006 Aug 24;4:29. |
| Genome Property | GenProp0748: choline ABC transporter, ChoXWV family (HMM-CLUST) |
| | GenProp0749: choline sulfate utilization (HMM-CLUST) |
