HMM Summary Page: TIGR03396

Functionphospholipase C, phosphocholine-specific
Trusted Cutoff580.25
Domain Trusted Cutoff580.25
Noise Cutoff465.55
Domain Noise Cutoff465.55
Isology Typeequivalog
EC Number3.1.4.3
HMM Length688
Mainrole CategoryCellular processes
Subrole CategoryPathogenesis
Gene Ontology TermGO:0004629: phospholipase C activity molecular_function
AuthorHaft DH
Entry DateApr 26 2007 10:35AM
Last ModifiedFeb 14 2011 3:27PM
CommentMembers of this protein family are bacterial, phosphatidylcholine-hydrolyzing phospholipase C enzymes, with a characteristic domain architecture as found in hemolytyic (PlcH) and nonhemolytic (PlcN) secreted enzymes of Pseudomonas aeruginosa. PlcH hydrolyzes phosphatidylcholine to diacylglycerol and phosphocholine, but unlike PlcN can also hydrolyze sphingomyelin to ceramide ((N-acylsphingosine)) and phosphocholine. Members of this family share the twin-arginine signal sequence for Sec-independent transport across the plasma membrane. PlcH is secreted as a heterodimer with a small chaperone, PlcR, encoded immediately downstream.
ReferencesRN [1] RM PMID: 12410824 RT A novel class of microbial phosphocholine-specific phospholipases C. RA Stonehouse MJ, Cota-Gomez A, Parker SK, Martin WE, Hankin JA, Murphy RC, Chen W, Lim KB, Hackett M, Vasil AI, Vasil ML RL Mol Microbiol. 2002 Nov;46(3):661-76. RN [2] RM PMID: 10523590 RT Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei. RA Korbsrisate S, Suwanasai N, Leelaporn A, Ezaki T, Kawamura Y, Sarasombath S RL J Clin Microbiol. 1999 Nov;37(11):3742-5.