HMM Summary Page: TIGR03283

Functionthymidylate synthase, methanogen type
Trusted Cutoff174.55
Domain Trusted Cutoff174.55
Noise Cutoff112.65
Domain Noise Cutoff112.65
Isology Typeequivalog
EC Number2.1.1.-
HMM Length201
Mainrole CategoryPurines, pyrimidines, nucleosides, and nucleotides
Subrole Category2'-Deoxyribonucleotide metabolism
Gene Ontology TermGO:0004799: thymidylate synthase activity molecular_function
GO:0009265: 2'-deoxyribonucleotide biosynthetic process biological_process
AuthorHaft DH
Entry DateJan 22 2007 12:08PM
Last ModifiedFeb 14 2011 3:27PM
CommentThymidylate synthase makes dTMP for DNA synthesis, and is among the most widely distributed of all enzymes. Members of this protein family are encoded within a completed genome sequence if and only if that species is one of the methanogenenic archaea. In these species, tetrahydromethanopterin replaces tetrahydrofolate, The member from Methanobacterium thermoautotrophicum was shown to behave as a thymidylate synthase based on similar side reactions (the exchange of a characteristic proton with water), although the full reaction was not reconstituted. Partial sequence data showed no similarity to known thymidylate synthases simply because the region sequenced was from a distinctive N-terminal region not found in other thymidylate synthases. Members of this protein family appear, therefore, to a novel, tetrahydromethanopterin-dependent thymidylate synthase.
ReferencesRN [1] RM PMID: 8143733 RT Purification and partial characterization of a putative thymidylate synthase from Methanobacterium thermoautotrophicum. RA Krone UE, McFarlan SC, Hogenkamp HP RL Eur J Biochem. 1994 Mar 15;220(3):789-94. DR HAMAP; MF_00008; 8 of 351