HMM Summary Page: TIGR03234

Functionhydroxypyruvate isomerase
Gene Symbolhyi
Trusted Cutoff353.45
Domain Trusted Cutoff353.45
Noise Cutoff349.15
Domain Noise Cutoff349.15
Isology Typeequivalog
EC Number5.3.1.22
HMM Length254
AuthorSelengut J
Entry DateJan 3 2007 11:02AM
Last ModifiedFeb 14 2011 3:27PM
CommentThis enzyme interconverts tartronate semi-aldehyde (TSA, aka 2-hydroxy 3-oxopropionate) and hydroxypyruvate. The E. coli enzyme has been characterized [1] and found to be specific for TSA, contain no cofactors, and have a rather high Km for hydroxypyruvate of 12.5 mM. The gene is ofter found in association with glyoxalate carboligase (which produces TSA), but has been shown to have no effect on growth on glyoxalate when knocked out [2]. This is consistent with the fact that the gene for tartronate semialdehyde reductase (glxR) is also associated and may have primary responsibility for the catabolism of TSA.
ReferencesRN [1] RM PMID: 10561547 RT Biochemical evidence that Escherichia coli hyi (orf b0508, gip) gene encodes hydroxypyruvate isomerase. RA Ashiuchi M, Misono H RL Biochim Biophys Acta. 1999 Nov 16;1435(1-2):153-9. RN [2] RM PMID: 8440684 RT Molecular cloning, DNA sequencing, and biochemical analyses of Escherichia coli glyoxylate carboligase. An enzyme of the acetohydroxy acid synthase-pyruvate oxidase family. RA Chang YY, Wang AY, Cronan JE Jr RL J Biol Chem. 1993 Feb 25;268(6):3911-9.
Genome PropertyGenProp0689: glyoxalate conversion to phosphoglycerate (HMM)