HMM Summary Page: TIGR03138

Functionqueuine synthase
Gene SymbolqueF
Trusted Cutoff293.45
Domain Trusted Cutoff293.45
Noise Cutoff223.60
Domain Noise Cutoff223.60
Isology Typeequivalog
EC Number1.7.1.13
HMM Length275
Mainrole CategoryTranscription
Subrole CategoryRNA processing
Gene Ontology TermGO:0008616: queuosine biosynthetic process biological_process
GO:0033739: preQ1 synthase activity molecular_function
GO:0043867: 7-cyano-7-deazaguanine tRNA-ribosyltransferase activity molecular_function
AuthorSelengut J
Entry DateNov 2 2006 3:31PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis enzyme catalyzes the 4-electron reduction of the cyano group of 7-cyano-7-deazaguanine (preQ0) to an amine [1]. Although related to a large family of GTP cyclohydrolases (PF01227), the relationship is structural and not germane to the catalytic mechanism. This mode represents the longer, gram-negative version of the enzyme as found in E. coli. The enzymatic step represents the first point at which the biosynthesis of queuosine in bacteria and eukaryotes is distinguished from the biosynthesis of archaeosine in archaea.
ReferencesRN [1] RM PMID: 15767583 RT From cyclohydrolase to oxidoreductase: discovery of nitrile reductase activity in a common fold. RA Van Lanen SG, Reader JS, Swairjo MA, de Crecy-Lagard V, Lee B, Iwata-Reuyl D RL Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4264-9. Epub 2005 Mar 14. DR HAMAP; MF_00817; 137 of 139
Genome PropertyGenProp0677: queuosine (Q-tRNA) biosynthesis from preQ0 (HMM)