HMM Summary Page: TIGR02884

Functiondelta-lactam-biosynthetic de-N-acetylase
Gene SymbolpdaA
Trusted Cutoff298.00
Domain Trusted Cutoff298.00
Noise Cutoff164.95
Domain Noise Cutoff164.95
Isology Typeequivalog
EC Number3.5.-.-
HMM Length224
Mainrole CategoryCell envelope
Subrole CategoryBiosynthesis and degradation of murein sacculus and peptidoglycan
AuthorHaft DH
Entry DateMar 10 2006 9:54AM
Last ModifiedFeb 14 2011 3:27PM
CommentMuramic delta-lactam is an unusual constituent of peptidoglycan, found only in bacterial spores in the peptidoglycan wall, or spore cortex. The proteins in this family are PdaA (yfjS), a member of a larger family of polysaccharide deacetylases, and are specificially involved in delta-lactam biosynthesis. PdaA acts immediately after CwlD, an N-acetylmuramoyl-L-alanine amidase and performs a de-N-acetylation. PdaA may also perform the following transpeptidation for lactam ring formation, as heterologous expression in E. coli of CwlD and PdaA together is sufficient for delta-lactam production.
ReferencesDR PFAM; PF01522; Polysacc_deac_1; RN [1] RM 14679227 RT Production of muramic delta-lactam in Bacillus subtilis spore peptidoglycan. RA Gilmore ME, Bandyopadhyay D, Dean AM, Linnstaedt SD, Popham DL. RL J Bacteriol. 2004 Jan;186(1):80-9. RN [2] RM 12374835 RT A polysaccharide deacetylase gene (pdaA) is required for germination and for production of muramic delta-lactam residues in the spore cortex of Bacillus subtilis. RA Fukushima T, Yamamoto H, Atrih A, Foster SJ, Sekiguchi J. RL J Bacteriol. 2002 Nov;184(21):6007-15.