HMM Summary Page: TIGR02686

Functionconjugative relaxase domain
Trusted Cutoff145.50
Domain Trusted Cutoff145.50
Noise Cutoff34.30
Domain Noise Cutoff34.30
Isology Typedomain
HMM Length286
Mainrole CategoryMobile and extrachromosomal element functions
Subrole CategoryPlasmid functions
Gene Ontology TermGO:0004386: helicase activity molecular_function
GO:0006259: DNA metabolic process biological_process
AuthorHaft DH
Entry DateNov 11 2005 3:59PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis domain is in the N-terminal (relaxase) region of TrwC, a relaxase-helicase that acts in plasmid R388 conjugation. The relaxase domain has DNA cleavage and strand transfer activities. Plasmid transfer protein TraI is also a member of this domain family. Members of this family on bacterial chromosomes typically are found near other genes typical of conjugative plasmids and appear to mark integrated plasmids.
ReferencesRN [1] RM 10653694 RT Two active-site tyrosyl residues of protein TrwC act sequentially at the origin of transfer during plasmid R388 conjugation. RA Grandoso G, Avila P, Cayon A, Hernando MA, Llosa M, de la Cruz F. RN [2] RM 12837798 RT A bacterial TrwC relaxase domain contains a thermally stable alpha-helical core. RA Arrondo JL, Echabe I, Iloro I, Hernando MA, de la Cruz F, Goni FM. RL J Bacteriol. 2003 Jul;185(14):4226-32.