| Accession | TIGR02506 |
| Name | NrdE_NrdA |
| Function | ribonucleoside-diphosphate reductase, alpha subunit |
| Trusted Cutoff | 341.05 |
| Domain Trusted Cutoff | 341.05 |
| Noise Cutoff | 319.00 |
| Domain Noise Cutoff | 319.00 |
| Isology Type | equivalog |
| EC Number | 1.17.4.1 |
| HMM Length | 616 |
| Mainrole Category | Purines, pyrimidines, nucleosides, and nucleotides |
| Subrole Category | 2'-Deoxyribonucleotide metabolism |
| Gene Ontology Term | GO:0005971: ribonucleoside-diphosphate reductase complex cellular_component |
| | GO:0009265: 2'-deoxyribonucleotide biosynthetic process biological_process |
| | GO:0016959: class I ribonucleotide reductase activity molecular_function |
| Author | Selengut J |
| Entry Date | Mar 7 2005 1:54PM |
| Last Modified | Feb 14 2011 3:27PM |
| Comment | This model represents the alpha (large) chain of the class I ribonucleotide reductase (RNR). RNR's are responsible for the conversion of the ribose sugar of RNA into the deoxyribose sugar of DNA. This is the rate-limiting step of DNA biosynthesis. Class I RNR's generate the required radical (on tyrosine) via a "non-heme" iron cofactor which resides in the beta (small) subunit. The alpha subunit contains the catalytic and allosteric regulatory sites. The mechanism of this enzyme requires molecular oxygen [1].
E. Coli contains two versions of this enzyme which are regulated independently (NrdAB and NrdEF, where NrdA and NrdE are the large chains [2,3]). Most organisms contain only one, but the application of the gene symbols NrdA and NrdE are somewhat arbitrary.
This model identifies RNR's in diverse clades of bacteria, eukaryotes as well as numerous DNA viruses and phage. |
| References | CC
CC
RN [1]
RM PMID: 11807048
RT Aerobic-type ribonucleotide reductase in the anaerobe Bacteroides fragilis.
RA Smalley D, Rocha ER, Smith CJ.
RL J Bacteriol. 2002 Feb;184(4):895-903.
RN [2]
RM PMID: 8052308
RT Structure of ribonucleotide reductase protein R1.
RA Uhlin U, Eklund H.
RL Nature. 1994 Aug 18;370(6490):533-9.
RN [3]
RM PMID: 8820648
RT Promoter identification and expression analysis of Salmonella typhimurium and Escherichia coli nrdEF operons encoding one of two class I ribonucleotide reductases present in both bacteria.
RA Jordan A, Aragall E, Gibert I, Barbe J.
RL Mol Microbiol. 1996 Feb;19(4):777-90. |
| Genome Property | GenProp0289: class I (aerobic) ribonucleotide reductase (HMM) |
