| Accession | TIGR02487 |
| Name | NrdD |
| Function | anaerobic ribonucleoside-triphosphate reductase |
| Gene Symbol | nrdD |
| Trusted Cutoff | 326.35 |
| Domain Trusted Cutoff | 326.35 |
| Noise Cutoff | 261.75 |
| Domain Noise Cutoff | 261.75 |
| Isology Type | equivalog |
| EC Number | 1.17.4.2 |
| HMM Length | 581 |
| Mainrole Category | Purines, pyrimidines, nucleosides, and nucleotides |
| Subrole Category | 2'-Deoxyribonucleotide metabolism |
| Gene Ontology Term | GO:0008998: ribonucleoside-triphosphate reductase activity molecular_function |
| | GO:0009265: 2'-deoxyribonucleotide biosynthetic process biological_process |
| Author | Selengut J |
| Entry Date | Feb 24 2005 12:08PM |
| Last Modified | Feb 14 2011 3:27PM |
| Comment | This model represents the oxygen-sensitive (anaerobic, class III) ribonucleotide reductase. The mechanism of the enzyme involves a glycine-centered radical [1], a C-terminal zinc binding site [2], and a set of conserved active site cysteines and asparagines [3]. This enzyme requires an activating component, NrdG, a radical-SAM domain containing enzyme (TIGR02491). Together the two form an alpha-2/beta-2 heterodimer. |
| References | RN [1]
RM PMID: 10066165
RT A glycyl radical site in the crystal structure of a class III ribonucleotide reductase.
RA Logan DT, Andersson J, Sjoberg BM, Nordlund P.
RL Science. 1999 Mar 5;283(5407):1499-504.
RN [2]
RM PMID: 12655046
RT A metal-binding site in the catalytic subunit of anaerobic ribonucleotide reductase.
RA Logan DT, Mulliez E, Larsson KM, Bodevin S, Atta M, Garnaud PE, Sjoberg BM, Fontecave M.
RL Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3826-31. Epub 2003 Mar 24.
RN [3]
RM PMID: 11526118
RT Two active site asparagines are essential for the reaction mechanism of the class III anaerobic ribonucleotide reductase from bacteriophage T4.
RA Andersson J, Bodevin S, Westman M, Sahlin M, Sjoberg BM.
RL J Biol Chem. 2001 Nov 2;276(44):40457-63. Epub 2001 Aug 28. |
| Genome Property | GenProp0291: class III (anaerobic) ribonucleotide reductase (HMM) |
