HMM Summary Page: TIGR02476

Function5,6-dimethylbenzimidazole synthase
Gene SymbolbluB
Trusted Cutoff150.00
Domain Trusted Cutoff150.00
Noise Cutoff120.00
Domain Noise Cutoff120.00
Isology Typeequivalog
EC Number1.14.99.40
HMM Length205
Gene Ontology TermGO:0009236: cobalamin biosynthetic process biological_process
GO:0016722: oxidoreductase activity, oxidizing metal ions molecular_function
AuthorHaft DH, Selengut J
Entry DateFeb 16 2005 4:18PM
Last ModifiedFeb 10 2012 10:31AM
CommentA previously published hypothesis that BluB, involved in cobalamin biosynthesis, is EC (cob(II)yrinic acid a,c-diamide reductase) is now contradicted by newer work ascribing a role in 5,6-dimethylbenzimidazole (DMB) biosynthesis. The BluB protein is related to the nitroreductase family (PF0881).
ReferencesRN [1] RM PMID:17301238 RT Single-enzyme conversion of FMNH2 to 5,6-dimethylbenzimidazole, the lower ligand of B12. RA Gray MJ, Escalante-Semerena JC RL Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2921-6. RN [2] RM PMID:17377583 RT BluB cannibalizes flavin to form the lower ligand of vitamin B12. RA Taga ME, Larsen NA, Howard-Jones AR, Walsh CT, Walker GC RL Nature. 2007 Mar 22;446(7134):449-53. RN [3] RM PMID: 12869542 RT Comparative genomics of the vitamin B12 metabolism and regulation in prokaryotes. RA Rodionov DA, Vitreschak AG, Mironov AA, Gelfand MS. RL J Biol Chem. 2003 Oct 17;278(42):41148-59. RN [4] RM PMID: 7635831 RT Identification and sequence analysis of genes involved in late steps in cobalamin (vitamin B12) synthesis in Rhodobacter capsulatus. RA Pollich M, Klug G. RL J Bacteriol. 1995 Aug;177(15):4481-7.