HMM Summary Page: TIGR02432

FunctiontRNA(Ile)-lysidine synthetase
Gene SymboltilS
Trusted Cutoff93.60
Domain Trusted Cutoff93.60
Noise Cutoff85.80
Domain Noise Cutoff85.80
Isology Typeequivalog_domain
EC Number6.3.4.-
HMM Length189
Mainrole CategoryProtein synthesis
Subrole CategorytRNA and rRNA base modification
Gene Ontology TermGO:0006400: tRNA modification biological_process
GO:0016879: ligase activity, forming carbon-nitrogen bonds molecular_function
AuthorHaft DH
Entry DateJan 18 2005 11:30AM
Last ModifiedFeb 14 2011 3:27PM
CommentThe only examples in which the wobble position of a tRNA must discriminate between G and A of mRNA are AUA (Ile) vs. AUG (Met) and UGA (stop) vs. UGG (Trp). In all bacteria, the wobble position of the tRNA(Ile) recognizing AUA is lysidine, a lysine derivative of cytidine. This family describes a protein domain found, apparently, in all bacteria in a single copy. Eukaryotic sequences appear to be organellar. The domain archictecture of this protein family is variable; some, including characterized proteins of E. coli and B. subtilis known to be tRNA(Ile)-lysidine synthetase, include a conserved 50-residue domain that many other members lack. This protein belongs to the ATP-binding PP-loop family ( PF01171). It appears in the literature and protein databases as TilS, YacA, and putative cell cycle protein MesJ (a misnomer).
ReferencesDR PFAM; PF01171; ATP_bind_3; PP-loop family RN [1] RM 14527414 RT An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA. RA Soma A, Ikeuchi Y, Kanemasa S, Kobayashi K, Ogasawara N, Ote T, Kato J, Watanabe K, Sekine Y, Suzuki T. RL Mol Cell. 2003 Sep;12(3):689-98. RN [2] RM 3132458 RT A novel lysine-substituted nucleoside in the first position of the anticodon of minor isoleucine tRNA from Escherichia coli. RA Muramatsu T, Yokoyama S, Horie N, Matsuda A, Ueda T, Yamaizumi Z, Kuchino Y, Nishimura S, Miyazawa T. RL J Biol Chem. 1988 Jul 5;263(19):9261-7. RN [3] RM 15044829 RT A global approach to identify novel broad-spectrum antibacterial targets among proteins of unknown function. RA Zalacain M, Biswas S, Ingraham KA, Ambrad J, Bryant A, Chalker AF, Iordanescu S, Fan J, Fan F, Lunsford RD, O'Dwyer K, Palmer LM, So C, Sylvester D, Volker C, Warren P, McDevitt D, Brown JR, Holmes DJ, Burnham MK. RL J Mol Microbiol Biotechnol. 2003;6(2):109-26. DR HAMAP; MF_01161; 237 of 240
Genome PropertyGenProp0799: bacterial core gene set, exactly 1 per genome (HMM)