HMM Summary Page: TIGR02415

Functionacetoin reductases
Trusted Cutoff304.35
Domain Trusted Cutoff304.35
Noise Cutoff242.75
Domain Noise Cutoff242.75
Isology Typesubfamily
EC Number1.1.1.-
HMM Length254
Mainrole CategoryEnergy metabolism
Subrole CategoryFermentation
AuthorHaft DH
Entry DateDec 28 2004 12:29PM
Last ModifiedFeb 14 2011 3:27PM
CommentOne member of this family, as characterized in Klebsiella terrigena, is described as able to interconvert acetoin + NADH with meso-2,3-butanediol + NAD(+). It is also called capable of irreversible reduction of diacetyl with NADH to acetoin. Blomqvist, et al. decline to specify either EC which is (R,R)-butanediol dehydrogenase, or EC, which is acetoin dehydrogenase without a specified stereochemistry, for this enzyme. This enzyme is a homotetramer in the family of short chain dehydrogenases (PF00106). Another member of this family, from Corynebacterium glutamicum, is called L-2,3-butanediol dehydrogenase (PMID:11577733).
ReferencesRN [1] RM 8444801 RT Characterization of the genes of the 2,3-butanediol operons from Klebsiella terrigena and Enterobacter aerogenes. RA Blomqvist K, Nikkola M, Lehtovaara P, Suihko ML, Airaksinen U, Straby KB, Knowles JK, Penttila ME. DR PFAM; PF00106; adh_short RN [2] RM 9332371 RT Molecular cloning and characterization of a cDNA encoding a bovine butanediol dehydrogenase. RA Smania AM, Argarana CE. RL Gene. 1997 Sep 15;197(1-2):231-8. RN [3] RM 11173520 RT Crystal structure of meso-2,3-butanediol dehydrogenase in a complex with NAD+ and inhibitor mercaptoethanol at 1.7 A resolution for understanding of chiral substrate recognition mechanisms. RA Otagiri M, Kurisu G, Ui S, Takusagawa Y, Ohkuma M, Kudo T, Kusunoki M. RL J Biochem (Tokyo). 2001 Feb;129(2):205-8. RN [4] RM 11577733 RT Purification and characterization of L-2,3-butanediol dehydrogenase of Brevibacterium saccharolyticum C-1012 expressed in Escherichia coli. RA Takusagawa Y, Otagiri M, Ui S, Ohtsuki T, Mimura A, Ohkuma M, Kudo T. RL Biosci Biotechnol Biochem. 2001 Aug;65(8):1876-8.