HMM Summary Page: TIGR02367
Accession | TIGR02367 |
Name | PylS_Cterm |
Function | pyrrolysine--tRNA ligase, C-terminal region |
Gene Symbol | pylS |
Trusted Cutoff | 178.15 |
Domain Trusted Cutoff | 178.15 |
Noise Cutoff | 177.85 |
Domain Noise Cutoff | 177.85 |
Isology Type | equivalog_domain |
EC Number | 6.1.1.26 |
HMM Length | 242 |
Mainrole Category | Protein synthesis |
Subrole Category | tRNA aminoacylation |
Gene Ontology Term | GO:0004812: aminoacyl-tRNA ligase activity molecular_function |
GO:0006418: tRNA aminoacylation for protein translation biological_process | |
Author | Haft DH |
Entry Date | Nov 2 2004 3:14PM |
Last Modified | Feb 14 2011 3:27PM |
Comment | PylS is the enzyme responsible for charging the pyrrolysine tRNA, PylT, by ligating a free molecule of pyrrolysine. Pyrrolysine is encoded at an in-frame UAG (amber) at least in several corrinoid-dependent methyltransferases of the archaeal genera Methanosarcina and Methanococcoides, such as trimethylamine methyltransferase. This protein occurs as a fusion protein in Methanosarcina but as split genes in Desulfitobacterium hafniense and other bacteria. |
References | RN [1] RM 15329732 RT Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo. RA Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA. RL Nature. 2004 Sep 16;431(7006):333-5. RN [2] RM 15314242 RT An aminoacyl-tRNA synthetase that specifically activates pyrrolysine. RA Polycarpo C, Ambrogelly A, Berube A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Soll D. RL Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12450-4. DR HAMAP; MF_01573; 6 of 6 |
Genome Property | GenProp0312: pyrrolysine (HMM) |