HMM Summary Page: TIGR02367

Functionpyrrolysine--tRNA ligase, C-terminal region
Gene SymbolpylS
Trusted Cutoff178.15
Domain Trusted Cutoff178.15
Noise Cutoff177.85
Domain Noise Cutoff177.85
Isology Typeequivalog_domain
EC Number6.1.1.26
HMM Length242
Mainrole CategoryProtein synthesis
Subrole CategorytRNA aminoacylation
Gene Ontology TermGO:0004812: aminoacyl-tRNA ligase activity molecular_function
GO:0006418: tRNA aminoacylation for protein translation biological_process
AuthorHaft DH
Entry DateNov 2 2004 3:14PM
Last ModifiedFeb 14 2011 3:27PM
CommentPylS is the enzyme responsible for charging the pyrrolysine tRNA, PylT, by ligating a free molecule of pyrrolysine. Pyrrolysine is encoded at an in-frame UAG (amber) at least in several corrinoid-dependent methyltransferases of the archaeal genera Methanosarcina and Methanococcoides, such as trimethylamine methyltransferase. This protein occurs as a fusion protein in Methanosarcina but as split genes in Desulfitobacterium hafniense and other bacteria.
ReferencesRN [1] RM 15329732 RT Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo. RA Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA. RL Nature. 2004 Sep 16;431(7006):333-5. RN [2] RM 15314242 RT An aminoacyl-tRNA synthetase that specifically activates pyrrolysine. RA Polycarpo C, Ambrogelly A, Berube A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Soll D. RL Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12450-4. DR HAMAP; MF_01573; 6 of 6
Genome PropertyGenProp0312: pyrrolysine (HMM)