HMM Summary Page: TIGR02258

Function2'-5' RNA ligase
Trusted Cutoff65.70
Domain Trusted Cutoff65.70
Noise Cutoff47.30
Domain Noise Cutoff47.30
Isology Typeequivalog
EC Number6.5.1.-
HMM Length180
Mainrole CategoryTranscription
Subrole CategoryRNA processing
Gene Ontology TermGO:0008033: tRNA processing biological_process
GO:0008664: 2'-5'-RNA ligase activity molecular_function
AuthorHaft DH
Entry DateAug 18 2004 5:08PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis protein family consists of bacterial and archaeal proteins with two tandem copies of Pfam domain PF02834. Members for which activity has been measured perform a reversible, ATP-independent 2'-5'-ligation of what is presumably a non-phyiological substrate: half-tRNA splice intermediates from an intron-containing yeast tRNA. The physiological substrate(s) in prokaryotes may include small 2'-5'-link-containing oligonucleotides, perhaps with regulatory or biosynthetic roles.
ReferencesDR PFAM; PF02834; 2_5_RNA_ligase RN [1] RM 8940112 RT The 2'-5' RNA ligase of Escherichia coli. Purification, cloning, and genomic disruption. RA Arn EA, Abelson JN. RL J Biol Chem. 1996 Dec 6;271(49):31145-53. RN [2] RM 12798681 RT Crystal structure of the 2'-5' RNA ligase from Thermus thermophilus HB8. RA Kato M, Shirouzu M, Terada T, Yamaguchi H, Murayama K, Sakai H, Kuramitsu S, Yokoyama S. RL J Mol Biol. 2003 Jun 20;329(5):903-11.