HMM Summary Page: TIGR02160

Functionphenylacetate-CoA oxygenase/reductase, PaaK subunit
Gene SymbolpaaK
Trusted Cutoff420.80
Domain Trusted Cutoff420.80
Noise Cutoff409.30
Domain Noise Cutoff409.30
Isology Typehypoth_equivalog
HMM Length352
Mainrole CategoryEnergy metabolism
Subrole CategoryOther
Gene Ontology TermGO:0010124: phenylacetate catabolic process biological_process
AuthorBrinkac L
Entry DateApr 13 2004 11:21AM
Last ModifiedFeb 14 2011 3:27PM
CommentPhenylacetate-CoA oxygenase is comprised of a five gene complex responsible for the hydroxylation of phenylacetate-CoA (PA-CoA) as the second catabolic step in phenylacetic acid (PA) degradation [1,2]. Although the exact function of this enzyme has not been determined, it has been shown to be required for phenylacetic acid degradation and has been proposed to function in a multicomponent oxygenase acting on phenylacetate-CoA [2].
ReferencesRN [1] RM 9600981 RT Molecular characterization of the phenylacetic acid catabolic pathway in Pseudomonas putida U: the phenylacetyl-CoA catabolon. RA Olivera ER, Minambres B, Garcia B, Muniz C, Moreno MA, Ferrandez A, Diaz E, Garcia JL, Luengo JM. RL Proc Natl Acad Sci U S A. 1998 May 26;95(11):6419-24. RN [2] RM 9748275 RT Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway. RA Ferrandez A, Minambres B, Garcia B, Olivera ER, Luengo JM, Garcia JL, Diaz E. RL J Biol Chem. 1998 Oct 2;273(40):25974-86. DR PFAM; PF00970; Oxidoreductase FAD-binding domain DR PFAM; PF00111; 2Fe-2S iron-sulfur cluster binding domain DR PFAM; PF00175; Oxidoreductase NAD-binding domain DR EXPERIMENTAL; GP|3253202; Pseudomonas putida; transposon mutagenesis prevented growth on MM containing PhAc, PhAc accumulation when grown in MM containing 4-OH-PhAc + PhAc and 4-OH-PhAc + PhH. DR EXPERIMENTAL; SP|P76077; Escherichia coli; transposon mutagenesis resulted in 2-HPA formation in MM containing glyucerol and PA. DR EXPERIMENTAL; GP|11072189; Azoarcus evansii; heterologous expression in E.coli with assay of soluable cell extracts. DR OUTGROUP; SP|P75824; Escherichia coli; NADH oxidoreductase hcr
Genome PropertyGenProp0210: phenylacetic acid catabolism (HMM)