HMM Summary Page: TIGR02055

Functionadenylylsulfate reductase, thioredoxin dependent
Trusted Cutoff235.30
Domain Trusted Cutoff235.30
Noise Cutoff232.80
Domain Noise Cutoff232.80
Isology Typesubfamily
EC Number1.8.4.10
HMM Length194
Mainrole CategoryCentral intermediary metabolism
Subrole CategorySulfur metabolism
Gene Ontology TermGO:0009973: adenylyl-sulfate reductase activity molecular_function
GO:0010134: sulfate assimilation via adenylyl sulfate reduction biological_process
AuthorBrinkac L
Entry DateNov 10 2003 2:12PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis model describes recently identified adenosine 5'-phosphosulfate (APS) reductase activity found in sulfate-assimilatory prokaryotes, thus separating it from the traditionally described phosphoadenosine 5'-phosphosulfate (PAPS) reductases found in bacteria and fungi [1]. Homologous to PAPS reductase in enterobacteria, cyanobacteria, and yeast, APS reductase here clusters with, and demonstrates greater homology to plant APS reductase. Additionally, the presence of two conserved C-terminal motifs (CCXXRKXXPL & SXGCXXCT) distinguishes APS substrate specificity and serves as a FeS cluster [2].
ReferencesRN [1] RM 10613872 RT Identification of a new class of 5'-adenylylsulfate (APS) reductases from sulfate-assimilating bacteria. RA Bick JA, Dennis JJ, Zylstra GJ, Nowack J, Leustek T. RL J Bacteriol. 2000 Jan;182(1):135-42. RN [2] RM 11940598 RT The presence of an iron-sulfur cluster in adenosine 5'-phosphosulfate reductase separates organisms utilizing adenosine 5'-phosphosulfate and phosphoadenosine 5'-phosphosulfate for sulfate assimilation. RA Kopriva S, Buchert T, Fritz G, Suter M, Benda R, Schunemann V, Koprivova A, Schurmann P, Trautwein AX, Kroneck PM, Brunold C. RL J Biol Chem. 2002 Jun 14;277(24):21786-91. Epub 2002 Apr 08. RN [3] RM 10464198 RT Reduction of adenosine-5'-phosphosulfate instead of 3'-phosphoadenosine-5'-phosphosulfate in cysteine biosynthesis by Rhizobium meliloti and other members of the family Rhizobiaceae. RA Abola AP, Willits MG, Wang RC, Long SR. RL J Bacteriol. 1999 Sep;181(17):5280-7. RN [4] RM 12072441 RT 5'-adenosinephosphosulfate lies at a metabolic branch point in mycobacteria. RA Williams SJ, Senaratne RH, Mougous JD, Riley LW, Bertozzi CR. RL J Biol Chem. 2002 Sep 6;277(36):32606-15. Epub 2002 Jun 18. DR PFAM; PF01507; Phosphoadenosine phosphosulfate reductase family DR EXPERIMENTAL; SP|P56891; Sinorhizobium meliloti; genetic complementation of E.coli mutants preferentiating APS rather than PAPS; kinetic properties consistent with Trx-dependent APS reductase activity. DR EXPERIMENTAL; SP|P94498; Bacillus subtilis; genetic complementation of E.coli mutants. DR EXPERIMENTAL; SP|P71752; Mycobacterium tuberculosis; genetic complementation of E.coli mutants and mutagenesis resulting in cysteine and methionine auxotrophy. DR EXPERIMENTAL; SP|O05927; Pseudomonas aeruginosa; genetic complemention of E.coli mutants and kinetic properties consistent with Trx-dependent APS reductase activity. DR OUTGROUP; SP|P17854; Escherichia coli; Phosphoadenosine phosphosulfate reductase. DR HAMAP; MF_00063; 30 of 92
Genome PropertyGenProp0149: sulfate reduction to sulfide, assimilatory (HMM)