HMM Summary Page: TIGR01718

Functionuridine phosphorylase
Gene Symboludp
Trusted Cutoff280.30
Domain Trusted Cutoff280.30
Noise Cutoff209.40
Domain Noise Cutoff209.40
Isology Typeequivalog
EC Number2.4.2.3
HMM Length245
Mainrole CategoryPurines, pyrimidines, nucleosides, and nucleotides
Subrole CategorySalvage of nucleosides and nucleotides
Gene Ontology TermGO:0004731: purine-nucleoside phosphorylase activity molecular_function
GO:0006139: nucleobase-containing compound metabolic process biological_process
AuthorSelengut J
Entry DateOct 4 2002 4:53PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis model represents a family of bacterial and archaeal uridine phosphorylases unrelated to the mammalian enzymes of the same name. The E. coli [1], Salmonella [2] and Klebsiella [3] genes have been characterized. Sequences from Clostridium, Streptomyces, Treponema, Halobacterium and Pyrobaculum were included above trusted on the basis of sequence homology and a PAM-based neighbor-joining tree. A clade including second sequences from Halobacterium and Vibrio was somewhat more distantly related and may represent a slightly different substrate specificity - these were placed below the noise cutoff. More distantly related is a clade of archaeal sequences which as related to the DeoD family of inosine phosphorylases (TIGR00107) as they are to these uridine phosphorylases. This clade includes a characterized protein from Sulfolobus solfataricus which has been mis-named as a methylthioadenosine phosphorylase, but which acts on inosine and guanosine - it is unclear whether uridine has been evaluated as a substrate [4].
ReferencesRN [1] RM PMID: 16751 RT Uridine phosphorylase from Escherichia coli. Physical and chemical characterization. RA Leer JC, Hammer-Jespersen K, Schwartz M. RL Eur J Biochem 1977 May 2;75(1):217-24 RN [2] RM PMID: 9526114 RT Isolation and initial characterization of the uridine phosphorylase from Salmonella typhimurium. RA Molchan OK, Dmitrieva NA, Romanova DV, Lopes LE, Debabov VG, Mironov AS. RL Biochemistry (Mosc) 1998 Feb;63(2):195-9 RN [3] RM PMID: 8534998 RT Molecular cloning and nucleotide sequence of purine nucleoside phosphorylase and uridine phosphorylase genes from Klebsiella sp. RA Takehara M, Ling F, Izawa S, Inoue Y, Kimura A. RL Biosci Biotechnol Biochem. 1995 Oct;59(10):1987-90. RN [4] RM PMID: 7929153 RT Purification and characterization of extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Purine nucleoside phosphorylase activity and evidence for intersubunit disulfide bonds. RA Cacciapuoti G, Porcelli M, Bertoldo C, De Rosa M, Zappia V. RL J Biol Chem. 1994 Oct 7;269(40):24762-9.