HMM Summary Page: TIGR01700

Functionpurine nucleoside phosphorylase I, inosine and guanosine-specific
Trusted Cutoff293.35
Domain Trusted Cutoff293.35
Noise Cutoff288.70
Domain Noise Cutoff288.70
Isology Typeequivalog
EC Number2.4.2.1
HMM Length249
Mainrole CategoryPurines, pyrimidines, nucleosides, and nucleotides
Subrole CategorySalvage of nucleosides and nucleotides
Gene Ontology TermGO:0004731: purine-nucleoside phosphorylase activity molecular_function
GO:0006139: nucleobase-containing compound metabolic process biological_process
AuthorSelengut J
Entry DateSep 24 2002 10:02PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis model represents a family of bacterial and metazoan purine phosphorylases acting primarily on inosine and guanosine and not acting on adenosine. PNP-I refers to the nomenclature from Bacillus stearothermophilus [1] where PHP-II refers to the nucleotidase acting on adenosine as the primary substrate. The bacterial enzymes (PUNA) are typified by the Bacilus PupG protein [2] which is involved in the metabolism of nucleosides as a carbon source. Several metazoan enzymes (PNPH) are well characterized including the human [3] and bovine [4] enzymes which have been crystallized.
ReferencesRN [1] RM PMID: 9058965 RT Cloning and expression of purine nucleoside phosphorylase I gene from Bacillus stearothermophilus TH 6-2. RA Hamamoto T, Okuyama K, Noguchi T, Midorikawa Y. RL Biosci Biotechnol Biochem. 1997 Feb;61(2):272-5. RN [2] RM PMID: 10537218 RT Nucleosides as a carbon source in Bacillus subtilis: characterization of the drm-pupG operon. RA Schuch R, Garibian A, Saxild HH, Piggot PJ, Nygaard P. RL Microbiology 1999 Oct;145 ( Pt 10):2957-66 RN [3] RM PMID: 2104852 RT Three-dimensional structure of human erythrocytic purine nucleoside phosphorylase at 3.2 A resolution. RA Ealick SE, Rule SA, Carter DC, Greenhough TJ, Babu YS, Cook WJ, Habash J, Helliwell JR, Stoeckler JD, Parks RE Jr, et al. RL J Biol Chem. 1990 Jan 25;265(3):1812-20. RN [4] RM PMID: 9020983 RT Crystal structure of calf spleen purine nucleoside phosphorylase in a complex with hypoxanthine at 2.15 A resolution. RA Koellner G, Luic M, Shugar D, Saenger W, Bzowska A. RL J Mol Biol. 1997 Jan 17;265(2):202-16.