| Accession | TIGR01670 |
| Name | KdsC-phosphatas |
| Function | 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family |
| Trusted Cutoff | 106.95 |
| Domain Trusted Cutoff | 106.95 |
| Noise Cutoff | 52.85 |
| Domain Noise Cutoff | 52.85 |
| Isology Type | subfamily |
| EC Number | 3.1.3.45 |
| HMM Length | 154 |
| Mainrole Category | Cell envelope |
| Subrole Category | Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides |
| Gene Ontology Term | GO:0009103: lipopolysaccharide biosynthetic process biological_process |
| | GO:0019143: 3-deoxy-manno-octulosonate-8-phosphatase activity molecular_function |
| Author | Selengut J |
| Entry Date | Sep 11 2002 11:10AM |
| Last Modified | Jan 23 2014 10:34AM |
| Comment | This family of proteins is a member of the IIIA subfamily of the haloacid dehalogenase (HAD) superfamily of hydrolases. All characterized members of this subfamily (TIGR01662) and most characterized members of the HAD superfamily are phosphatases. HAD superfamily phosphatases contain active site residues in several conserved catalytic motifs [1], all of which are found conserved here.
One member of this family, the YrbI protein from H. influenzae has been cloned, expressed, purified and found to be an active 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase[2]. Furthermore, its crystal structure has been determined [3].
This family consists of sequences from beta, gamma and epsilon proteobacteria, Aquifex, Fusobacterium, Porphyromonas and Methanosarcina.
The Methanosarcina sequence is distinctive in that it is linked to an N-terminal cytidylyltransferase domain (PF02348) and is annotated as acylneuraminate cytidylyltransferase. This may give some clue as the function of these phosphatases. Several eukaryotic sequences scoring between trusted and noise are also closely related to this function such as the CMP-N-acetylneuraminic acid synthetase from mouse, but in these cases the phosphatase domain is clearly inactive as many of the active site residues are not conserved. |
| References | RN [1]
RM PMID: 7966317
RT Computer analysis of bacterial haloacid dehalogenases defines a large superfamily of hydrolases with diverse specificity. Application of an iterative approach to database search.
RA Koonin EV, Tatusov RL.
RL J Mol Biol 1994 Nov 18;244(1):125-32
RN [2]
RM PMID: 12639950
RT Escherichia coli YrbI is 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase.
RA Wu J, Woodard RW.
RL J Biol Chem. 2003 May 16;278(20):18117-23. Epub 2003 Mar 14.
RN [3]
RM PMID: 11835514
RT From structure to function: YrbI from Haemophilus influenzae (HI1679) is a phosphatase.
RA Parsons JF, Lim K, Tempczyk A, Krajewski W, Eisenstein E, Herzberg O.
RL Proteins 2002 Mar 1;46(4):393-404 |
| Genome Property | GenProp0204: KDO(2)-lipid A (Re LPS) biosynthesis and delivery (HMM) |
