| Accession | TIGR01531 |
| Name | glyc_debranch |
| Function | glycogen debranching enzyme |
| Gene Symbol | GDE |
| Trusted Cutoff | 1399.55 |
| Domain Trusted Cutoff | 1399.55 |
| Noise Cutoff | 574.95 |
| Domain Noise Cutoff | 574.95 |
| Isology Type | equivalog |
| HMM Length | 1443 |
| Mainrole Category | Energy metabolism |
| Subrole Category | Biosynthesis and degradation of polysaccharides |
| Gene Ontology Term | GO:0004133: glycogen debranching enzyme activity molecular_function |
| | GO:0005980: glycogen catabolic process biological_process |
| Author | Durkin S |
| Entry Date | Jun 5 2002 12:06PM |
| Last Modified | Feb 14 2011 3:27PM |
| Comment | glycogen debranching enzyme possesses two different catalytic activities; oligo-1,4-->1,4-glucantransferase (EC 2.4.1.25) and amylo-1,6-glucosidase (EC 3.2.1.33). Site directed mutagenesis studies in S. cerevisiae [1] indicate that the transferase and glucosidase activities are independent and located in different regions of the polypeptide chain. Proteins in this model belong to the larger alpha-amylase family. The model covers eukaryotic proteins with a seed composed of human, nematode and yeast sequences. Yeast seed sequence is well characterized. The model is quite rigorous; either query sequence yields large bit score or it fails to hit the model altogether. There doesn't appear to be any middle ground. |
| References | RN [1]
RM PMID: 11375985
RT Identification of the catalytic residues of bifunctional glycogen debranching enzyme.
RA Nakayama A, Yamamoto K, Tabata S
RL J Biol Chem 2001 Aug 3;276(31):28824-8
RN [2]
RM PMID: 8136030
RT Starch- and glycogen-debranching and branching enzymes: prediction of structural features of the catalytic (beta/alpha)8-barrel domain and evolutionary relationship to other amylolytic enzymes
RA Jespersen HM, MacGregor EA, Henrissat B, Sierks MR, Svensson B
RL J Protein Chem 1993 Dec;12(6):791-805 |
| Genome Property | GenProp0168: glycogen system (HMM) |
