HMM Summary Page: TIGR01486

AccessionTIGR01486
NameHAD-SF-IIB-MPGP
Functionmannosyl-3-phosphoglycerate phosphatase family
Trusted Cutoff164.75
Domain Trusted Cutoff164.75
Noise Cutoff114.15
Domain Noise Cutoff114.15
Isology Typesubfamily
EC Number3.1.3.-
HMM Length257
AuthorSelengut J, Haft DH
Entry DateApr 11 2002 1:53PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis small group of proteins is a member of the IIB subfamily (TIGR01484) of the Haloacid Dehalogenase (HAD) superfamily of aspartate-nucleophile hydrolases. Several members of this family from thermophiles (and from Dehalococcoides ethenogenes) are now known to act as mannosyl-3-phosphoglycerate (MPG) phosphatase. In these cases, the enzyme acts after MPG synthase to make the compatible solute mannosylglycerate. We propose that other mesophilic members of this family do not act as mannosyl-3-phosphoglycerate phosphatase. A member of this family is found in Escherichia coli, which appears to lack MPG synthase. Mannosylglycerate is imported in E. coli by phosphoenolpyruvate-dependent transporter (PMID:14645248), but it appears the phosphorylation is not on the glycerate moiety, that the phosphorylated import is degraded by an alpha-mannosidase from an adjacent gene, and that E. coli would have no pathway to obtain MPG.
ReferencesRM 12788726 RT The bacterium Thermus thermophilus, like hyperthermophilic archaea, uses a two-step pathway for the synthesis of mannosylglycerate. RA Empadinhas N, Albuquerque L, Henne A, Santos H, da Costa MS. RL Appl Environ Microbiol. 2003 Jun;69(6):3272-9. DR HAMAP; MF_00617; 18 of 19