HMM Summary Page: TIGR01454

FunctionAHBA synthesis associated protein
Trusted Cutoff246.65
Domain Trusted Cutoff246.65
Noise Cutoff132.25
Domain Noise Cutoff132.25
Isology Typehypoth_equivalog
HMM Length205
AuthorSelengut J
Entry DateFeb 28 2002 3:42PM
Last ModifiedFeb 14 2011 3:27PM
CommentThe enzymes in this equivalog are all located in the operons for the biosynthesis of 3-amino-5-hydroxybenoic acid (AHBA), which is a precursor of several antibiotics including ansatrienin [1], naphthomycin [1], rifamycin [2] and mitomycin [3]. The role that this enzyme plays in this biosynthesis has not been elucidated. This enzyme is a member of the Haloacid dehalogenase superfamily (PF00702) of aspartate-nucleophile hydrolases. This enzyme is closely related to phosphoglycolate phosphatase (TIGR01449), but it is unclear what purpose a PGPase or PGPase-like activity would serve in these biosyntheses. This model is limited to the Gram positive Actinobacteria. The most closely related enzyme below the noise cutoff is IndB which is involved in the biosynthesis of Indigoidine in Pectobacterium (Erwinia) chrysanthemi, a gamma proteobacter [4]. This enzyme is similarly related to PGP. In this case, too it is unclear what role would be be played by a PGPase activity.
ReferencesRN [1] RM PMID: 10103039 RT Biosynthesis of ansatrienin (mycotrienin) and naphthomycin. Identification and analysis of two separate biosynthetic gene clusters in Streptomyces collinus Tu 1892. RA Chen S, von Bamberg D, Hale V, Breuer M, Hardt B, Muller R, Floss HG, Reynolds KA, Leistner E. RL Eur J Biochem 1999 Apr;261(1):98-107 RN [2] RM PMID: 11278540 RT Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. RA Yu TW, Muller R, Muller M, Zhang X, Draeger G, Kim CG, Leistner E, Floss HG. RL J Biol Chem 2001 Apr 20;276(16):12546-55 RN [3] RM PMID: 10099135 RT Molecular characterization and analysis of the biosynthetic gene cluster for the antitumor antibiotic mitomycin C from Streptomyces lavendulae NRRL 2564. RA Mao Y, Varoglu M, Sherman DH. RL Chem Biol 1999 Apr;6(4):251-63 RN [4] RM PMID: 11790734 RT Characterization of indigoidine biosynthetic genes in Erwinia chrysanthemi and role of this blue pigment in pathogenicity. RA Reverchon S, Rouanet C, Expert D, Nasser W. RL J Bacteriol 2002 Feb;184(3):654-65 DR PFAM; PF00702; hydrolase; Haloacid dehalogenase superfamily