| Accession | TIGR01369 |
| Name | CPSaseII_lrg |
| Function | carbamoyl-phosphate synthase, large subunit |
| Gene Symbol | carB |
| Trusted Cutoff | 1027.00 |
| Domain Trusted Cutoff | 1027.00 |
| Noise Cutoff | 469.10 |
| Domain Noise Cutoff | 469.10 |
| Isology Type | equivalog_domain |
| EC Number | 6.3.5.5 |
| HMM Length | 1052 |
| Mainrole Category | Purines, pyrimidines, nucleosides, and nucleotides |
| Subrole Category | Pyrimidine ribonucleotide biosynthesis |
| Gene Ontology Term | GO:0004088: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity molecular_function |
| | GO:0005951: carbamoyl-phosphate synthase complex cellular_component |
| | GO:0009220: pyrimidine ribonucleotide biosynthetic process biological_process |
| Author | Haft DH |
| Entry Date | Oct 30 2001 10:37AM |
| Last Modified | Feb 14 2011 3:27PM |
| Comment | Carbamoyl-phosphate synthase (CPSase) catalyzes the first committed step in pyrimidine, arginine, and urea biosynthesis. In general, it is a glutamine-dependent enzyme, EC 6.3.5.5, termed CPSase II in eukaryotes. An exception is the mammalian mitochondrial urea-cycle form,
CPSase I, in which the glutamine amidotransferase domain active site Cys on the small subunit has been lost, and the enzyme is ammonia-dependent. In both CPSase I and the closely related, glutamine-dependent CPSase III (allosterically activated by acetyl-glutamate) demonstrated in some other vertebrates, the small and large chain regions are fused in a single polypeptide chain. This HMM represents the large chain of glutamine-hydrolysing carbamoyl-phosphate synthases, or the corresponding regions of larger, multifunctional proteins, as found in all domains of life, and CPSase I forms are considered exceptions within the family.
In several thermophilic species (Methanobacterium thermoautotrophicum, Methanococcus jannaschii, Aquifex aeolicus), the large subunit appears split, at different points, into two separate genes. |
| References | DR EXCEPTION; SP|P31327; EC 6.3.4.16; Homo sapiens
DR EXPERIMENTAL; SP|P00968; Escherichia coli
DR HAMAP; MF_01210; 171 of 173 |
| Genome Property | GenProp0187: pyrimidine (uridine-5'-phosphate) de novo biosynthesis (HMM) |
