HMM Summary Page: TIGR01298

Functionribonuclease T
Gene Symbolrnt
Trusted Cutoff246.20
Domain Trusted Cutoff246.20
Noise Cutoff60.20
Domain Noise Cutoff60.20
Isology Typeequivalog
EC Number3.1.13.-
HMM Length200
Mainrole CategoryTranscription
Subrole CategoryRNA processing
Gene Ontology TermGO:0006364: rRNA processing biological_process
GO:0016896: exoribonuclease activity, producing 5'-phosphomonoesters molecular_function
GO:0042780: tRNA 3'-end processing biological_process
AuthorHaft DH
Entry DateJul 2 2001 12:22PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis HMM describes ribonuclease T, an enzyme found so far only in gamma-subdivision Proteobacteria such as Escherichia coli and Xylella fastidiosa. Ribonuclease T is homologous to the DNA polymerase III alpha chain. It can liberate AMP from the common C-C-A terminus of uncharged tRNA. It appears also to be involved in RNA maturation. It also acts as a 3' to 5' single-strand DNA-specific exonuclease; it is distinctive for its ability to remove residues near a double-stranded stem. Ribonuclease T is a high copy suppressor in E. coli of a uv-repair defect caused by deletion of three other single-stranded DNA exonucleases.
ReferencesRN [1] RM 9857048 RT Identification of a potent DNase activity associated with RNase T of Escherichia coli. RA Viswanathan M, Dower KW, Lovett ST. RL J Biol Chem 1998 Dec 25;273(52):35126-31 DR HAMAP; MF_00157; 80 of 81