HMM Summary Page: TIGR01290

Functionnitrogenase cofactor biosynthesis protein NifB
Gene SymbolnifB
Trusted Cutoff343.65
Domain Trusted Cutoff343.65
Noise Cutoff222.20
Domain Noise Cutoff222.20
Isology Typeequivalog
HMM Length441
Mainrole CategoryBiosynthesis of cofactors, prosthetic groups, and carriers
Subrole CategoryOther
Gene Ontology TermGO:0009399: nitrogen fixation biological_process
GO:0051186: cofactor metabolic process biological_process
AuthorHaft DH
Entry DateJun 26 2001 3:50PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis HMM describes NifB, a protein required for the biosynthesis of the iron-molybdenum (or iron-vanadium) cofactor used by the nitrogen-fixing enzyme nitrogenase. NifB belongs to the radical SAM family, and the FeMo cluster biosynthesis process requires S-adenosylmethionine. Archaeal homologs lack the most C-terminal region and score between the trusted and noise cutoffs of this model.
ReferencesRN [1] RM 7592933 RT Incorporation of iron and sulfur from NifB cofactor into the iron-molybdenum cofactor of dinitrogenase. RA Allen RM, Chatterjee R, Ludden PW, Shah VK RL J Biol Chem 1995 Nov 10;270(45):26890-6 RN [2] RM 10364262 RT A vanadium and iron cluster accumulates on VnfX during iron-vanadium-cofactor synthesis for the vanadium nitrogenase in Azotobacter vinelandii. RA Ruttimann-Johnson C, Staples CR, Rangaraj P, Shah VK, Ludden PW RL J Biol Chem 1999 Jun 18;274(25):18087-92 RN [3] RM PMID:16567617 RT NifB-dependent in vitro synthesis of the iron-molybdenum cofactor of nitrogenase. RA Curatti L, Ludden PW, Rubio LM RL Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5297-301.
Genome PropertyGenProp0029: nitrogen fixation (HMM)