HMM Summary Page: TIGR01125

Functionribosomal protein S12 methylthiotransferase RimO
Gene SymbolrimO
Trusted Cutoff356.95
Domain Trusted Cutoff356.95
Noise Cutoff332.15
Domain Noise Cutoff332.15
Isology Typeequivalog
EC Number2.1.1.- 2.8.1.-
HMM Length432
Mainrole CategoryProtein synthesis
Subrole CategorytRNA and rRNA base modification
Gene Ontology TermGO:0006400: tRNA modification biological_process
GO:0016782: transferase activity, transferring sulfur-containing groups molecular_function
AuthorHaft DH, Selengut J
Entry DateJan 16 2001 2:22PM
Last ModifiedFeb 14 2011 3:27PM
CommentMembers of this protein are the methylthiotransferase RimO, which modifies a conserved Asp residue in ribosomal protein S12. This clade of radical SAM family proteins is closely related to the tRNA modification bifunctional enzyme MiaB (see TIGR01574), and it catalyzes the same two types of reactions: a radical-mechanism sulfur insertion, and a methylation of the inserted sulfur. This clade spans alpha and gamma proteobacteria, cyano bacteria, Deinococcus, Porphyromonas, Aquifex, Helicobacter, Campylobacter, Thermotoga, Chlamydia, Streptococcus coelicolor and Clostridium, but does not include most other gram positive bacteria, archaea or eukaryotes.
ReferencesRN [1] RM PMID:19736993 RT Characterization of RimO, a new member of the methylthiotransferase subclass of the radical SAM superfamily. RA Lee KH, Saleh L, Anton BP, Madinger CL, Benner JS, Iwig DF, Roberts RJ, Krebs C, Booker SJ RL Biochemistry. 2009 Oct 27;48(42):10162-74. RN [2] RM PMID:20007320 RT Post-translational modification of ribosomal proteins: structural and functional characterization of RimO from Thermotoga maritima, a radical-SAM methylthiotransferase. RA Arragain S, Garcia-Serres R, Blondin G, Douki T, Clemancey M, Latour JM, Forouhar F, Neely H, Montelione GT, Hunt JF, Mulliez E, Fontecave M, Atta M RL J Biol Chem. 2009 Dec 9.