HMM Summary Page: TIGR00768

Functionalpha-L-glutamate ligase, RimK family
Trusted Cutoff143.40
Domain Trusted Cutoff143.40
Noise Cutoff100.80
Domain Noise Cutoff100.80
Isology Typesubfamily
EC Number6.3.2.-
HMM Length276
AuthorHaft DH
Entry DateJun 13 2000 5:57PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis family, related to bacterial glutathione synthetases, contains at least three different alpha-L-glutamate ligases. One is RimK, as in E. coli, which adds additional Glu residues to the native Glu-Glu C-terminus of ribosomal protein S6, but not to Lys-Glu mutants. Most species with a member of this subfamily lack an S6 homolog ending in Glu-Glu, however. Members in Methanococcus jannaschii act instead as a tetrahydromethanopterin:alpha-l-glutamate ligase (MJ0620) and a gamma-F420-2:alpha-l-glutamate ligase (MJ1001).
ReferencesAL clustalw DR EXPERIMENTAL; EGAD|6445|EC0852; Escherichia coli RN [1] RM 89364710 RT Characterization of the gene rimK responsible for the addition of glutamic acid residues to the C-terminus of ribosomal protein S6 in Escherichia coli K12. RA Kang WK, Icho T, Isono S, Kitakawa M, Isono K RL Mol Gen Genet 1989 Jun;217(2-3):281-8 RN [2] RM 98078575 RT A diverse superfamily of enzymes with ATP-dependent carboxylate-amine/thiol ligase activity. RA Galperin MY, Koonin EV RL Protein Sci 1997 Dec;6(12):2639-43 RN [3] RT Glutathione synthetase homologs encode alpha-L-glutamate ligases for methanogenic coenzyme F420 and tetrahydrosarcinapterin biosyntheses. RA Li H, Xu H, Graham DE, White RH. RL Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9785-90 DR HAMAP; MF_01552; 114 of 117