HMM Summary Page: TIGR00748

Functionputative hydroxymethylglutaryl-CoA synthase
Trusted Cutoff353.35
Domain Trusted Cutoff353.35
Noise Cutoff353.15
Domain Noise Cutoff353.15
Isology Typeequivalog
EC Number2.3.3.10
HMM Length345
Mainrole CategoryFatty acid and phospholipid metabolism
Subrole CategoryBiosynthesis
Gene Ontology TermGO:0004315: 3-oxoacyl-[acyl-carrier-protein] synthase activity molecular_function
GO:0006633: fatty acid biosynthetic process biological_process
AuthorSelengut J, Haft DH
Entry DateMar 2 2000 2:33PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis family of archaeal proteins shows considerable homology and identical active site residues to the bacterial hydroxymethylglutaryl-CoA synthase (HMG-CoA synthase, modeled by TIGR01835) which is the second step in the mevalonate pathway of IPP biosynthesis [1]. An enzyme from Pseudomonas fluorescens involved in the biosynthesis of the polyketide diacetyl-phloroglucinol is more closely related, but lacks the active site residues [2]. In each of the genomes containing a member of this family there is no other recognized HMG-CoA synthase, although other elements of the mevalonate pathway are in evidence. The only archaeon currently sequenced which lacks a homolog in this pathway is Halobacterium, which _does_ contain a separate HMG-CoA synthase. Thus, although there is no experimental evidence supporting this name, the bioinformatics-based conclusion appears to be sound.
ReferencesRN [1] RM PMID: 12107122 RT Enterococcus faecalis 3-hydroxy-3-methylglutaryl coenzyme A synthase, an enzyme of isopentenyl diphosphate biosynthesis. RA Sutherlin A, Hedl M, Sanchez-Neri B, Burgner JW 2nd, Stauffacher CV, Rodwell VW. RL J Bacteriol. 2002 Aug;184(15):4065-70. RN [2] RM PMID: 10322017 RT Identification and characterization of a gene cluster for synthesis of the polyketide antibiotic 2,4-diacetylphloroglucinol from Pseudomonas fluorescens Q2-87. RA Bangera MG, Thomashow LS. RL J Bacteriol. 1999 May;181(10):3155-63. DR HAMAP; MF_01409; 30 of 30
Genome PropertyGenProp0047: IPP biosynthesis via mevalonate (HMM)