HMM Summary Page: TIGR00683

FunctionN-acetylneuraminate lyase
Gene SymbolnanA
Trusted Cutoff382.00
Domain Trusted Cutoff382.00
Noise Cutoff154.10
Domain Noise Cutoff154.10
Isology Typeequivalog
EC Number4.1.3.3
HMM Length293
Mainrole CategoryCell envelope
Subrole CategoryBiosynthesis and degradation of surface polysaccharides and lipopolysaccharides
Gene Ontology TermGO:0006040: amino sugar metabolic process biological_process
GO:0008747: N-acetylneuraminate lyase activity molecular_function
GO:0009273: peptidoglycan-based cell wall biogenesis biological_process
AuthorHaft DH
Entry DateJan 13 2000 2:40PM
Last ModifiedFeb 14 2011 3:27PM
CommentN-acetylneuraminate lyase is also known as N-acetylneuraminic acid aldolase, sialic acid aldolase, or sialate lyase. It is an intracellular enzyme. The structure of this homotetrameric enzyme related to dihydrodipicolinate synthase is known. In Clostridium tertium, the enzyme appears to be in an operon with a secreted sialidase that releases sialic acid from host sialoglycoconjugates. In several E. coli strains, however, this enzyme is responsible for N-acetyl-D-neuraminic acid synthesis for capsule production by condensing N-acetyl-D-mannosamine and pyruvate.
ReferencesRN [1] RM 98205111 RT Expression in Escherichia coli of the putative N-acetylneuraminate lyase gene (nanA) from Haemophilus influenzae: overproduction, purification, and crystallization. RA Lilley GG, Barbosa JA, Pearce LA RL Protein Expr Purif 1998 Apr;12(3):295-304 RN [2] RM 99054726 DR EXPERIMENTAL; EGAD|29522|EC3225; Escherichia coli DR EXPERIMENTAL; EGAD|27817|HI0142; Haemophilus influenzae DR EXPERIMENTAL; EGAD|150412|160512; Trichomonas vaginalis DR OUTGROUP; EGAD|14906|EC2478; dihydrodipicolinate synthase DR HAMAP; MF_01237; 30 of 49