HMM Summary Page: TIGR00639

Functionphosphoribosylglycinamide formyltransferase
Gene SymbolpurN
Trusted Cutoff150.10
Domain Trusted Cutoff150.10
Noise Cutoff127.00
Domain Noise Cutoff127.00
Isology Typeequivalog
EC Number2.1.2.2
HMM Length192
Mainrole CategoryPurines, pyrimidines, nucleosides, and nucleotides
Subrole CategoryPurine ribonucleotide biosynthesis
Gene Ontology TermGO:0004644: phosphoribosylglycinamide formyltransferase activity molecular_function
GO:0009152: purine ribonucleotide biosynthetic process biological_process
AuthorHaft DH
Entry DateOct 25 1999 11:40AM
Last ModifiedFeb 14 2011 3:27PM
CommentThis HMM describes phosphoribosylglycinamide formyltransferase (GAR transformylase), one of several proteins in formyl_transf (Pfam family PF00551). This enzyme uses formyl tetrahydrofolate as a formyl group donor to produce 5'-phosphoribosyl-N-formylglycinamide. PurT, a different GAR transformylase, uses ATP and formate rather than formyl tetrahydrofolate. Experimental proof includes complementation of E. coli purN mutants by orthologs from vertebrates (where it is a domain of a multifunctional protein), Bacillus subtilis, and Arabidopsis. No archaeal example was detected. In phylogenetic analyses, the member from Saccharomyces cerevisiae shows a long branch length but membership in the family, while the formyltetrahydrofolate deformylases form a closely related outgroup.
ReferencesA2 hmmalign GA hmmsearch DR PFAM; PF00551; formyl_transf DR YPD; ADE8 DR OUTGROUP; EGAD|15350|EC1232 DR OUTGROUP; EGAD|156449|AA1257 RM 87280114 RT Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12. RA Smith JM, Daum HA 3d RL J Biol Chem 1987 Aug 5;262(22):10565-9
Genome PropertyGenProp0110: purine (inosine-5'-phosphate) biosynthesis from ribose-5-phosphate (HMM)