HMM Summary Page: TIGR00581

Functionmolybdenum cofactor biosynthesis protein C
Gene SymbolmoaC
Trusted Cutoff141.50
Domain Trusted Cutoff141.50
Noise Cutoff71.45
Domain Noise Cutoff71.45
Isology Typeequivalog
HMM Length148
Mainrole CategoryBiosynthesis of cofactors, prosthetic groups, and carriers
Subrole CategoryMolybdopterin
Gene Ontology TermGO:0003824: catalytic activity molecular_function
GO:0006777: Mo-molybdopterin cofactor biosynthetic process biological_process
AuthorHaft DH
Entry DateOct 19 1999 6:19PM
Last ModifiedFeb 14 2011 3:27PM
CommentMoaC catalyzes an early step in molybdenum cofactor biosynthesis in E. coli. The Arabidopsis homolog Cnx3 complements MoaC deficiency in E. coli (MUID:95197640). Eukarotic members of this family branch within the bacterial branch, with the archaeal members as an apparent outgroup. This protein is absent in a number of the pathogens with smaller genomes, including Mycoplasmas, Chlamydias, and spirochetes, but is found in most other complete genomes to date. The homolog form Synechocystis sp. is fused to a MobA-homologous region and is an outlier to all other bacterial forms by both neighbor-joining and UPGMA analyses. Members of this family are well-conserved. The seed for this model excludes both archaeal sequences and the most divergent bacterial sequences, but still finds all candidate MoaC sequences easily between trusted and noise cutoffs. We suggest that sequences branching outside the set that contains all seed members be regarded only as putative functional equivalents of MoaC unless and until a member of the archaeal outgroup is shown to have equivalent function.
ReferencesGA hmmsearch RN [1] RM 95197640 RT Isolation of two Arabidopsis cDNAs involved in early steps of molybdenum cofactor biosynthesis by functional complementation of Escherichia coli mutants. RA Hoff T, Schnorr KM, Meyer C, Caboche M RL J Biol Chem 1995 Mar 17;270(11):6100-7 DR OUTGROUP; EGAD|105404|AF2150 DR OUTGROUP; EGAD|43752|MJ1135 DR EXPERIMENTAL; EGAD|21351|EC0783; DR EXPERIMENTAL; EGAD|25607|26408; DR HAMAP; MF_01224; 228 of 237
Genome PropertyGenProp0466: molybdopterin biosynthesis (HMM)