HMM Summary Page: TIGR00563

Function16S rRNA (cytosine(967)-C(5))-methyltransferase
Gene SymbolrsmB
Trusted Cutoff269.05
Domain Trusted Cutoff269.05
Noise Cutoff188.00
Domain Noise Cutoff188.00
Isology Typeequivalog
EC Number2.1.1.176
HMM Length437
Mainrole CategoryProtein synthesis
Subrole CategorytRNA and rRNA base modification
Gene Ontology TermGO:0000154: rRNA modification biological_process
GO:0016434: rRNA (cytosine) methyltransferase activity molecular_function
AuthorHaft DH
Entry DateOct 19 1999 4:31PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis protein is also known as sun protein. The reading frame was originally interpreted as two reading frames, fmu and fmv. The recombinant protein from E. coli was shown to methylate only C967 of small subunit (16S) ribosomal RNA and to produce only m5C at that position. The seed alignment is built from bacterial sequences only. Eukaryotic homologs include Nop2, a protein required for processing pre-rRNA, that is likely also a rRNA methyltransferase, although the fine specificity may differ. Cutoff scores are set to avoid treating archaeal and eukaroytic homologs automatically as functionally equivalent, although they may have very similar roles.
ReferencesA2 hmmalign DR PFAM; PF01189; NOL1/NOP2/sun family DR PROSITE; PDOC00888; NOL1/NOP2/sun family signature RM 99152378 RT Purification, cloning, and characterization of the 16S RNA m5C967 methyltransferase from Escherichia coli. RA Tscherne JS, Nurse K, Popienick P, Michel H, Sochacki M, Ofengand J RL Biochemistry 1999 Feb 9;38(6):1884-92