HMM Summary Page: TIGR00520

FunctionL-asparaginase, type II
Trusted Cutoff314.30
Domain Trusted Cutoff314.30
Noise Cutoff211.35
Domain Noise Cutoff211.35
Isology Typesubfamily
EC Number3.5.1.1
HMM Length352
Mainrole CategoryEnergy metabolism
Subrole CategoryAmino acids and amines
AuthorHaft DH
Entry DateOct 18 1999 5:03PM
Last ModifiedFeb 21 2012 12:13PM
CommentTwo related families of asparaginase (L-asparagine amidohydrolase, EC are designated type I and type II according to the terminology in E. coli, which has both: L-asparaginase I is a low-affinity enzyme found in the cytoplasm, while L-asparaginase II is a high-affinity periplasmic enzyme synthesized with a cleavable signal sequence. This model describes L-asparaginases related to type II of E. coli. Both the cytoplasmic and the cell wall asparaginases of Saccharomyces cerevisiae belong to this set. Members of this set from Acinetobacter glutaminasificans and Pseudomonas fluorescens are described as having both glutaminase and asparaginase activitities. All members are homotetrameric.
ReferencesA2 hmmalign AL clustalw_manual RM 90170867 RT Analysis of the Escherichia coli gene encoding L-asparaginase II, ansB, and its regulation by cyclic AMP receptor and FNR proteins. RA Jennings MP, Beacham IR RL J Bacteriol 1990 Mar;172(3):1491-8 DR PFAM; PF00710; Asparaginase DR PROSITE; PDOC0013; Asparaginase / glutaminase active sites signatures DR ECOCYC; EG10046; ansB