HMM Summary Page: TIGR00342

FunctiontRNA sulfurtransferase ThiI
Gene SymbolthiI
Trusted Cutoff264.50
Domain Trusted Cutoff264.50
Noise Cutoff166.10
Domain Noise Cutoff166.10
Isology Typeequivalog_domain
EC Number2.8.1.4
HMM Length373
Mainrole CategoryBiosynthesis of cofactors, prosthetic groups, and carriers
Subrole CategoryThiamine
Gene Ontology TermGO:0003824: catalytic activity molecular_function
GO:0006400: tRNA modification biological_process
GO:0009228: thiamine biosynthetic process biological_process
AuthorLoftus BJ, Haft DH
Entry DateApr 20 1999 2:05PM
Last ModifiedJan 25 2012 12:12PM
CommentMembers of this protein family are "ThiI", a sulfurtransferase involved in 4-thiouridine modification of tRNA. This protein often is bifunctional, with genetically separable activities, where the C-terminal rhodanese-like domain (residues 385 to 482 in E. coli ThiI), a domain not included in this model, is sufficient to synthesize the thiazole moiety of thiamine (see TIGR04271). Note that ThiI, because of its role in tRNA modification, may occur in species (such as Mycoplasma genitalium) that lack de novo thiamine biosynthesis.
ReferencesRN [1] RM PMID:21724998 RT The rhodanese domain of ThiI is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica. RA Martinez-Gomez NC, Palmer LD, Vivas E, Roach PL, Downs DM RL J Bacteriol. 2011 Sep;193(18):4582-7. RN [2] RM PMID:18604845 RT Direct evidence that ThiI is an ATP pyrophosphatase for the adenylation of uridine in 4-thiouridine biosynthesis. RA You D, Xu T, Yao F, Zhou X, Deng Z RL Chembiochem. 2008 Aug 11;9(12):1879-82. RN [3] RM 11443125 RT The role of the cysteine residues of ThiI in the generation of 4-thiouridine in tRNA. RA Mueller EG, Palenchar PM, Buck CJ. RL J Biol Chem. 2001 Sep 7;276(36):33588-95. RN [4] RM 97352701 RT Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium. RA Webb E, Claas K, Downs DM RL J Bacteriol 1997 Jul;179(13):4399-402 DR PFAM; PF02568 DR InterPro; IPR003720 DR HAMAP; MF_00021; 184 of 191