HMM Summary Page: TIGR00329

Functionmetallohydrolase, glycoprotease/Kae1 family
Trusted Cutoff207.80
Domain Trusted Cutoff207.80
Noise Cutoff124.95
Domain Noise Cutoff124.95
Isology Typesubfamily
HMM Length305
Mainrole CategoryProtein fate
Subrole CategoryDegradation of proteins, peptides, and glycopeptides
AuthorLoftus BJ, Haft DH
Entry DateApr 20 1999 2:07PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis subfamily includes the well-studied secreted O-sialoglycoprotein endopeptidase (glycoprotease, EC of Pasteurella haemolytica, a pathogen. A member from Riemerella anatipestifer, associated with cohemolysin activity, likewise is exported without benefit of a classical signal peptide and shows glycoprotease activity on the test substrate glycophorin. However, archaeal members of this subfamily show unrelated activities as demonstrated in Pyrococcus abyssi: DNA binding, iron binding, apurinic endonuclease activity, genomic association with a kinase domain, and no glycoprotease activity. This family thus pulls together a set of proteins as a homology group that appears to be near-universal in life, yet heterogeneous in assayed function between bacteria and archaea.
ReferencesDR PROSITE; PDOC00779; GLYCOPROTEASE AL clustalw, belvu DR HAMAP; MF_01447; 21 of 21 RN [1] RM PMID:1885539 RT Cloning, nucleotide sequence, and expression of the Pasteurella haemolytica A1 glycoprotease gene. RA Abdullah KM, Lo RY, Mellors A RL J Bacteriol. 1991 Sep;173(18):5597-603. RN [2] RM PMID:17766251 RT An archaeal orthologue of the universal protein Kae1 is an iron metalloprotein which exhibits atypical DNA-binding properties and apurinic-endonuclease activity in vitro. RA Hecker A, Leulliot N, Gadelle D, Graille M, Justome A, Dorlet P, Brochier C, Quevillon-Cheruel S, Le Cam E, van Tilbeurgh H, Forterre P RL Nucleic Acids Res. 2007;35(18):6042-51. RN [3] RM PMID:11889100 RT Identification and characterization of CAMP cohemolysin as a potential virulence factor of Riemerella anatipestifer. RA Crasta KC, Chua KL, Subramaniam S, Frey J, Loh H, Tan HM RL J Bacteriol. 2002 Apr;184(7):1932-9.