HMM Summary Page: TIGR00324

FunctiontRNA-intron lyase
Gene SymbolendA
Trusted Cutoff59.60
Domain Trusted Cutoff59.60
Noise Cutoff47.60
Domain Noise Cutoff47.60
Isology Typeequivalog
EC Number4.6.1.16
HMM Length177
Mainrole CategoryTranscription
Subrole CategoryRNA processing
Gene Ontology TermGO:0000213: tRNA-intron endonuclease activity molecular_function
GO:0000214: tRNA-intron endonuclease complex cellular_component
GO:0006388: tRNA splicing, via endonucleolytic cleavage and ligation biological_process
AuthorLoftus BJ
Entry DateApr 20 1999 2:07PM
Last ModifiedJul 7 2014 1:34PM
CommentThe enzyme catalyses the endonucleolytic cleavage of pre tRNA at the 5' and 3' splice sites to release the intron and produces two half tRNA molecules bearing 5' hydroxyl and 2', 3'-cyclic phosphate termini. The genes are homologous in Eucarya and Archea. The two yeast genes have been functionally studied (see MEDLINE; 97344075) and are two subunits of a heterotetramer enzyme in yeast the other two subunits of which have no known homologs.
ReferencesRM 97344075 RT Properties of H. volcanii tRNA intron endonuclease reveal a relationship between the archaeal and eucaryal tRNA intron processing systems. RA Kleman-Leyer K, Armbruster DW, Daniels CJ RL Cell 1997 Jun 13;89(6):839-47 SE TIGR GA hmmls AL clustalw DR HAMAP; MF_01834; 9 of 12