HMM Summary Page: TIGR00134

Functionglutamyl-tRNA(Gln) amidotransferase, subunit E
Gene SymbolgatE
Trusted Cutoff319.55
Domain Trusted Cutoff319.55
Noise Cutoff223.40
Domain Noise Cutoff223.40
Isology Typeequivalog
EC Number6.3.5.-
HMM Length622
Mainrole CategoryProtein synthesis
Subrole CategorytRNA aminoacylation
Gene Ontology TermGO:0006424: glutamyl-tRNA aminoacylation biological_process
GO:0050567: glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity molecular_function
AuthorHaft DH
Entry DateApr 20 1999 2:07PM
Last ModifiedFeb 14 2011 3:27PM
CommentThis peptide is found only in the Archaea. It is paralogous to the gatB-encoded subunit of Glu-tRNA(Gln) amidotransferase. The GatABC system operates in many bacteria to convert Glu-tRNA(Gln) into Gln-tRNA(Gln). However, the homologous system in archaea instead converts Asp-tRNA(Asn) to Asn-tRNA(Asn). Glu-tRNA(Gln) is converted to Gln-tRNA(Gln) by a heterodimeric amidotransferase of GatE (this protein) and GatD. The Archaea have an Asp-tRNA(Asn) amidotransferase instead of an Asp--tRNA ligase, but the genes have not been identified. It is likely that this protein replaces gatB in Asp-tRNA(Asn) amidotransferase but that both enzymes share gatA.
ReferencesDR PROSITE; PDOC00948; DR PFAM; PF01162; PET112 family, C terminal region RM 10993083 RT Domain-specific recruitment of amide amino acids for protein synthesis. RA Tumbula DL, Becker HD, Chang WZ, Soll D. RL Nature. 2000 Sep 7;407(6800):106-10. DR HAMAP; MF_00588; 29 of 29
Genome PropertyGenProp0258: tRNA aminoacylation (HMM)
GenProp0831: archaeal core gene set, exactly 1 per genome (HMM)