| Accession | TIGR00118 |
| Name | acolac_lg |
| Function | acetolactate synthase, large subunit, biosynthetic type |
| Gene Symbol | ilvB |
| Trusted Cutoff | 538.35 |
| Domain Trusted Cutoff | 538.35 |
| Noise Cutoff | 489.00 |
| Domain Noise Cutoff | 489.00 |
| Isology Type | equivalog |
| EC Number | 2.2.1.6 |
| HMM Length | 557 |
| Mainrole Category | Amino acid biosynthesis |
| Subrole Category | Pyruvate family |
| Gene Ontology Term | GO:0003984: acetolactate synthase activity molecular_function |
| | GO:0005948: acetolactate synthase complex cellular_component |
| | GO:0009097: isoleucine biosynthetic process biological_process |
| | GO:0009099: valine biosynthetic process biological_process |
| Author | Haft DH |
| Entry Date | Apr 20 1999 2:06PM |
| Last Modified | Feb 14 2011 3:27PM |
| Comment | Two groups of proteins form acetolactate from two molecules of pyruvate. The type of acetolactate synthase described in this model also catalyzes the formation of acetohydroxybutyrate from pyruvate and 2-oxobutyrate, an early step in the branched chain amino acid biosynthesis; it is therefore also termed acetohydroxyacid synthase.
In bacteria, this catalytic chain is associated with a smaller regulatory chain in an alpha2/beta2 heterotetramer.
Acetolactate synthase is a thiamine pyrophosphate enzyme. In this type, FAD and Mg++ are also found.
Several isozymes of this enzyme are found in E. coli K12, one of which contains a frameshift in the large subunit gene and is not expressed. |
| References | A2 hmmalign
DR PROSITE; PDOC00166;
DR PFAM; PF00205; TPP_enzymes;
RN [1]
RM 97254452
RT Cloning and phylogenetic analysis of the genes encoding acetohydroxyacid synthase from the archaeon Methanococcus aeolicus.
RN [2]
RM 98322107
RT Biosynthesis of 2-aceto-2-hydroxy acids: acetolactate synthases and acetohydroxyacid synthases.
RA Chipman D, Barak Z, Schloss JV;
RL Biochim Biophys Acta 1998 Jun 29;1385(2):401-19
SE TIGR
GA hmmls
AL clustalw, belvu
DR OUTGROUP; SP:P30146; glyoxylate carboligase; E. coli |
| Genome Property | GenProp0162: isoleucine biosynthesis from threonine and pyruvate (HMM) |
| | GenProp0163: valine biosynthesis from pyruvate (HMM) |
| | GenProp0164: leucine biosynthesis from pyruvate and acetyl-CoA (HMM) |
