HMM Summary Page: TIGR00013

Function4-oxalocrotonate tautomerase family enzyme
Gene SymboldmpI
Trusted Cutoff56.90
Domain Trusted Cutoff56.90
Noise Cutoff31.50
Domain Noise Cutoff31.50
Isology Typesubfamily
EC Number5.3.2.-
HMM Length64
Mainrole CategoryEnergy metabolism
Subrole CategoryOther
Gene Ontology TermGO:0006725: cellular aromatic compound metabolic process biological_process
GO:0016862: intramolecular oxidoreductase activity, interconverting keto- and enol-groups molecular_function
AuthorDodson RJ, Richardson DL
Entry DateApr 20 1999 2:10PM
Last ModifiedFeb 14 2011 3:27PM
Comment4-oxalocrotonate tautomerase is a homohexamer in which each monomer is very small, at about 62 amino acids. Pro-1 of the mature protein serves as a general base. The enzyme functions in meta-cleavage pathways of aromatic hydrocarbon catabolism. Because several Arg residues located near the active site in the crystal structure of Pseudomonas putida are not conserved among all members of this family, because the literature describes a general role in the isomerization of beta,gamma-unsaturated enones to their alpha,beta-isomers, and because of the presence of fairly distantly related paralogs in Campylobacter jejuni, the family is regarded as not necessarily uniform in function.
ReferencesDR EXPERIMENAL; SP|Q01468; Pseudomonas putida RN [1] RM 12051677 RT The 4-oxalocrotonate tautomerase family of enzymes: how nature makes new enzymes using a beta-alpha-beta structural motif. RA Whitman CP. RL Arch Biochem Biophys 2002 Jun 1;402(1):1-13 RN [2] RM 11329265 RT The structural basis for the perturbed pKa of the catalytic base in 4-oxalocrotonate tautomerase: kinetic and structural effects of mutations of Phe-50. RA Czerwinski RM, Harris TK, Massiah MA, Mildvan AS, Whitman CP. RL Biochemistry 2001 Feb 20;40(7):1984-95
Genome PropertyGenProp0708: catechol meta-cleavage pathway (HMM)