HMM Summary Page: TIGR00010

Functionhydrolase, TatD family
Trusted Cutoff206.00
Domain Trusted Cutoff206.00
Noise Cutoff108.85
Domain Noise Cutoff108.85
Isology Typesubfamily
HMM Length253
Mainrole CategoryUnknown function
Subrole CategoryEnzymes of unknown specificity
Gene Ontology TermGO:0008152: metabolic process biological_process
GO:0016787: hydrolase activity molecular_function
AuthorHaft DH, Richardson DL, Hickey E
Entry DateFeb 22 2000 11:31AM
Last ModifiedFeb 14 2011 3:27PM
CommentPSI-BLAST, starting with a urease alpha subunit, finds a large superfamily of proteins, including a number of different enzymes that act as hydrolases at C-N bonds other than peptide bonds (EC 3.5.-.-), many uncharacterized proteins, and the members of this family. Several genomes have multiple paralogs related to this family. However, a set of 17 proteins can be found, one each from 17 of the first 20 genomes, such that each member forms a bidirectional best hit across genomes with all other members of the set. This core set (and one other near-perfect member), but not the other paralogs, form the seed for this model. Additionally, members of the seed alignment and all trusted hits, but not all paralogs, have a conserved motif DxHxH near the amino end. The member from E. coli was recently shown to have DNase activity.
ReferencesSE TIGR AL clustalw_manual RM 10747959 RT TatD is a cytoplasmic protein with DNase activity. No requirement for TatD family proteins in sec-independent protein export. RA Wexler M, Sargent F, Jack RL, Stanley NR, Bogsch EG, Robinson C, Berks BC, Palmer T. RL J Biol Chem 2000 Jun 2;275(22):16717-22