HMM Summary Page: TIGR00004

Functionreactive intermediate/imine deaminase
Trusted Cutoff106.25
Domain Trusted Cutoff106.25
Noise Cutoff80.80
Domain Noise Cutoff80.80
Isology Typesubfamily
HMM Length125
Mainrole CategoryTranscription
Subrole CategoryDegradation of RNA
Gene Ontology TermGO:0004521: endoribonuclease activity molecular_function
GO:0006402: mRNA catabolic process biological_process
AuthorDodson RJ, Richardson DL, Haft DH
Entry DateApr 20 1999 2:04PM
Last ModifiedMay 22 2014 4:42PM
CommentThis protein was described initially as an inhibitor of protein synthesis intiation, then as an endoribonuclease active on single-stranded mRNA, endoribonuclease L-PSP. Members of this family, conserved in all domains of life and often with several members per bacterial genome, appear to catalyze a reaction that minimizes toxic by-products from reactions catalyzed by pyridoxal phosphate-dependent enzymes.
ReferencesRN [1] RM PMID:22094463 RT Conserved YjgF protein family deaminates reactive enamine/imine intermediates of pyridoxal 5'-phosphate (PLP)-dependent enzyme reactions. RA Lambrecht JA, Flynn JM, Downs DM RL J Biol Chem. 2012 Jan 27;287(5):3454-61. RN [2] RM 10400702 RT Ribonuclease Activity of Rat Liver Perchloric Acid-soluble Protein, a Potent Inhibitor of Protein Synthesis. RA Morishita R, Kawagoshi A, Sawasaki T, Madin K, Ogasawara T, Oka T, Endo Y RL J Biol Chem 1999 Jul 16;274(29):20688-20692 RN [3] RM 96102067 RT Isolation and characterization of a novel perchloric acid-soluble protein inhibiting cell-free protein synthesis. RA Oka T, Tsuji H, Noda C, Sakai K, Hong YM, Suzuki I, Munoz S, Natori Y RL J Biol Chem 1995 Dec 15;270(50):30060-7 RN [4] RM 99296592 RT A role for a highly conserved protein of unknown function in regulation of Bacillus subtilis purA by the purine repressor. RA Rappu P, Shin BS, Zalkin H, Mantsala P RL J Bacteriol 1999 Jun;181(12):3810-5 RN [5] RM PMID:23749972 RT In the absence of RidA, endogenous 2-aminoacrylate inactivates alanine racemases by modifying the pyridoxal 5'-phosphate cofactor. RA Flynn JM, Downs DM RL J Bacteriol. 2013 Aug;195(16):3603-9. doi: 10.1128/JB.00463-13.